BOTTLE,20191212CSICIIMFFP
# Data from Fiz F Perez via DIGITAL CSIC 12/12/2010
# Ship: Mytilus
# Cruise: DYBAGA
# EXPOCODE 29MY20010515
# Dates: 2001-05-15 - 2002-04-24 (47 repetitions)
# Region: Off NW Spain 

License:  The authors appreciate that users of these data: 1) Contact Fiz F. Perez (fiz.perez@iim.csic.es) or Anton Velo (avelo@iim.csic.es) to follow the uses of the data, and 2) Include the requested acknowledgment (cite using the DOI of this dataset) in any presentations or publications.

Authors: Fiz F. Pérez, M. Nieto-Cid, X.A. Alvarez-Salgado, C.G. Castro, G. Roson, W. Redondo, M. Gilcoto y Aida F. Rios. DYBAGA: Weekly hydrographic dataset on SW Galician Shelf. Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC). 

# Chief Scientist - Fiz F. Perez
# 3 stations * 47 repetitions with CTD SBE 9 with 12 10L Niskin bottle
# Hydro: Who -Miguel Gil Coto - final 
# Notes: Cast fabricated/ Station numbers= 100*Repetition +(0,3 or 5)
# Bottom depth approximated from global topography
# Nuts/O2: Who -Alvarez-Salgado/Carmen Castro/Fiz F Perez; Status - final     
# Notes:  Winkler titration Pt electrode micromol/kg
# Nitrate Autoanalizer ALPKEM using Cd-Cu reduction (Mouriño and Fraga 1985)  micromol/L
# Phosphate Autoanalizer Hansen and Grasshof (1983)  micromol/L
# Silicate Autoanalizer Hansen and Grasshof (1983)   micromol/L
# TA: Who - Gago/Marta Alvarez/Perez; Status - final 
# Notes: precision 0.1%; accuracy 2 micromol/kg (Perez and Fraga 1987 ) (CRM)
# pH: Who - Gago/Perez; Status - final   
# Notes: pHT at 25 DEGC  (Clayton and Byrne 1993) +0.0047 (DelValls and Dickson, 1998)
# Spectrophotmetric at 25 DEGC
# CHLORA fluoremetrically Turner 10000R 
# Particulate organic matter and dissolved organic matter also included. Measured simultaneously nitrogen-specific Antek 7020 nitric oxide chemiluminescence detector, coupled with the carbon infrared gas analyser of a Shimadzu TOC-5000 (Álvarez-Salgado and Miller 1998)
# References: 
# http://dx.doi.org/10.1029/2004JC002473  Alvarez-Salgado et al 2006 Stoichiometry of the degradation of dissolved and particulate biogenic,
# http://dx.doi.org/10.1016/j.dsr.2006.09.002 Castro et al. 2006 Local remineralization patterns in the mesopelagic zone of the ENAW
# http://dx.doi.org/10.3354/meps283039  Nieto-Cid et al. 2004 Cycling of dissolved and particulate carbohydrates in a coastal upwelling system (NW Iberian Peninsula)
 Name            Units
 _________    ____________    
STNNBR	
CASTNO	
BTLNBR	
BTLNBR_FLAG_W	
DATE	         'aaaaammdd' 
LATITUDE	     'degrees'
LONGITUDE	     'degrees'
BOTTOM DEPTH     'meters'
DEPTH	         'meters'  
CTDPRS	         'DeciBARS'
CTDTMP	         'ITS-90'
SALNTY	         'PSS-78'
SALNTY_FLAG_W	
OXYGEN	         'micromol/kg'
OXYGEN_FLAG_W	
SILCAT           'microg/L'
SILCAT_FLAG_W	
NITRAT           'microg/L'
NITRAT_FLAG_W	
NITRIT           'microg/L'
NITRIT_FLAG_W	
PHSPHT           'microg/L'
PHSPHT_FLAG_W	
ALKALI           'micromol/kg'
ALKALI_FLAG_W
PH_NBS	         'pH units'
PH_TEMP	         'DEGC'
PH_NBS_FLAG_W	
AMMONIA          'microg/kg'
AMMONIA_FLAG
CHLORA	         'microg/L'
CHLORA_FLAG_W	
PON	             'micromol/L'
PON_FLAG	 
POC	             'micromol/L'
POC_FLAG	
POP	             'micromol/L'
POP_FLAG
DON	             'micromol/L'
DON_FLAG	 
DOC	             'micromol/L'
DOC_FLAG	
DOP	             'micromol/L'
DOP_FLAG		
THETA	         'DEGC'    


DESCRIPTION

For one year, the variability at weekly frequency of the phenomena of upwelling and downwelling in the Galician shelf in front of the Ria of Vigo and its impact on the different biogeochemical and carbon cycle variables were studied. The relative importance of physical and biological processes in the variability of pCO2 was evaluated. The net production rates of carbon species in the ecosystem (DIC, POC, DOC) were determined to evaluate the relative importance of accumulation versus export of dissolved organic fractions and newly formed particles. The field work also developed the study of elemental and biochemical compositions of (1) organic matter dissolved in seawater, (2) soft and hard particles suspended in water and (3) biogenic materials that have been mineralized in the water column of the NW Iberian upwelling system.  
Four stations were sampled weekly from May 2001 to April 2002 between the shelf-break (1200 m depth) to the middle of the Ria de Vigo (45 m depth). Salinity and temperature were recorded with a SBE 9/11 conductivity-temperature depth probe attached to the rosette sampler. Salinity and temperature were recorded with a SBE 9/11 conductivity/temperature depth probe connected to the rosette sampler with twelve 10 L Niskin PVC bottles with internal stainless steel springs. Conductivity measurements were converted into practical values of the salinity scale with the UNESCO equation (1986). The accuracy of CTD measurements for temperature and salinity were 0.004 DEGC and 0.005, respectively. For the determination of dissolved oxygen, samples were collected directly in calibrated 110 mL glass flasks and, after fixation with Cl2Mn and NaOH/NaI, kept in the dark until analysis in the laboratory 24 hours later. Oxygen was determined by potentiometric titration of the Winkler endpoint using a Titrino 720 (Metrohm) analyser with an accuracy of 0.5 micromol/kg. 
For nutrient salts determination, water samples were collected in 50 ml polyethylene bottles and kept cold (4 DEGC) until analysis in the laboratory using standard segmented flow analysis procedures. Precisions were 0.02 microM for nitrite, 0.1 microM for nitrate, 0.05 microM for ammonium, 0.02 microM for phosphate and 0.05 microM for silicate. 
Total alkalinity (TA) and pH (total hydrogen concentration scale, 25 DEGC) samples were collected in 500 ml glass vials and analyzed in a few hours in the base laboratory. Seawater pH was measured spectrophotometrically following Clayton and Byrne (1993) with an addition of 0.0047 (DelValls and Dickson, 1998). The precision was 0.003 pH units. The TA was determined by titration at pH 4.4 with HCl, according to the potentiometric method of Perez and Fraga (1987) with a precision of 2 micromol/kg.
Suspended organic matter was collected under low vacuum on precombusted (450 DEGC, 4 hours) 25 mm diameter Whatman GF/F filters of 0.7 micrometer nominal pore size (POC/PON, 0.5–1.5 L of seawater). All filters were dried overnight and frozen (-20 DEGC) before analysis. POC and PON measurements were made with a PerkinElmer 2400 CHN analyzer. An acetanilide standard was used daily. The precision of the method is 0.3 microM for carbon and 0.1 microM for nitrogen. The determination of particulate organic phosphorus (POP) followed the same collection and storage procedures as for POC/PON, after filtration of 250 ml of seawater. The particulate matter collected in Whatman's GF/F filters was determined by H2SO4/HClO4 digestion at 220°C. The phosphoric acid produced was analysed, after neutralisation, by the SFA procedure for phosphate. The phosphate standards were applied every day of analysis. The precision for the entire analysis was 0.02 microM.
Dissolved organic matter samples weres collected in 500 ml acid-cleaned flasks and filtered through precombusted (450 DEGC, 4 hours) 47 mm diameter Whatman GF/F filters (nominal pore size, 0.7 mm) in an acid-cleaned glass filtration system, under low N2 flow pressure. Aliquots for the analysis of DOC/DON were collected into 10 mL precombusted (450 DEGC, 12 hours) glass ampoules. After acidification with H3PO4 to pH<2, the ampoules were heat-sealed and stored in the dark at 4 DEGC until analysis. DOC and DON were measured simultaneously with a nitrogen-specific Antek 7020 nitric oxide chemiluminescence detector coupled in series with the carbon specific Infrared Gas Analyzer of a Shimadzu TOC-5000 organic carbon analyzer (Alvarez-Salgado and Miller, 1998). The precision is 0.7 microM for carbon and 0.2 microM for nitrogen.  Samples for the determination of dissolved organic phosphorus were collected and filtered as indicated for DOC/DON samples. The filtrate was collected in 50 mL polyethylene containers and frozen at -20°C until analysis. It was measured by the SFA phosphate system after oxidation with Na2S2O8/borax and UV radiation (Armstrong et al., 1966). Only monophosphoric organic esters are analysed, as polyphosphates are resistant to this oxidation procedure. Daily calibrations with phosphate, phenyl phosphate and adenosine 5'-monophosphate (AMP) in of seawater. MPA standards were analysed to calculate the recovery of monophosphoric esters (about 80%). The precision of the method was estimated at 0.04 microM.

Data available at https://digital.csic.es/