Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/99990
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Characterization of the anaerobic microbial community in oil-polluted subtidal sediments: Aromatic biodegradation potential after the Prestige oil spill

AutorAcosta-González, Alejandro CSIC ORCID; Rosselló-Mora, Ramón CSIC ORCID; Marqués, Silvia CSIC ORCID
Fecha de publicación2013
EditorBlackwell Publishing
CitaciónEnvironmental Microbiology 15(1):77-92 (2013)
ResumenThe influence of massive crude oil contamination on the microbial population of coastal sediments was investigated in the Cíes Islands 18 and 53 months after the tanker Prestige sank off the NW coast of Spain. Communities were studied by means of culturable and non-culturable methods at three horizons in the sediment (2-5cm, 12-15cm and 25-30cm) in an area heavily affected by the spill. Most probable number of aerobic hydrocarbon degraders was highest in the upper zone and decreased dramatically with depth. Aromatic oxidizing nitrate-reducing bacteria counts were slightly higher than aerobes in the oxidized layer, and also decreased considerably with depth. Iron-reducing bacteria were barely detectable. The highest counts were obtained for sulfate-reducing bacteria, which represented the most relevant fraction of aromatic oxidizers, being maximal at 12-15cm depth. The community response to high pollution levels was characterized by an increase in culturable populations active towards crude oil components despite the strong decay in the total cell counts. Analysis of whole 16S rRNA gene libraries obtained from the two sampling times and different depths (1460 sequences in all) showed a predominance of Gamma- and Deltaproteobacteria, which was confirmed by fluorescent in situ hybridization. Desulfobacteraceae was the most abundant group among Deltaproteobacteria, followed by sequences affiliated with the order Myxococcales. All retrieved sequences of this order affiliated with a marine myxobacterial clade. Interestingly, sequences affiliated to the order Desulfarculales constituted half of the Deltaproteobacteria sequences retrieved from the heaviest contaminated sample. Principal coordinates analysis of 16S rRNA gene libraries suggested fluctuation in the community distribution with time. Changes in the abundance of certain groups such as Bacteroidetes contributed to these observed differences. Although predominance of certain metabolic types in each horizon could be delimited, a considerable overlap in the use of electron acceptors was observed, confirming that each selected zone could be influenced by more than one respiratory metabolism. Altogether, our results evidence the presence in these sediments of a microbial community with potential to respond against hydrocarbon contamination, consistent with the long pollution history of the site. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Versión del editorhttp://dx.doi.org/10.1111/j.1462-2920.2012.02782.x
URIhttp://hdl.handle.net/10261/99990
DOI10.1111/j.1462-2920.2012.02782.x
Identificadoresdoi: 10.1111/j.1462-2920.2012.02782.x
issn: 1462-2912
Aparece en las colecciones: (IMEDEA) Artículos
(EEZ) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

112
checked on 08-mar-2024

WEB OF SCIENCETM
Citations

109
checked on 22-feb-2024

Page view(s)

305
checked on 19-mar-2024

Download(s)

113
checked on 19-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.