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Continuous production of xylooligosaccharides in a packed bed reactor with immobilized-stabilized biocatalysts of xylanase from Aspergillus versicolor

AuthorsAragon, Caio C.; Santos, Andrea F.; Ruiz-Matute, Ana I. ; Corzo, Nieves ; Guisán, José Manuel ; Monti, Rubens; Mateo González, César
KeywordsEnzyme thermostabilization
Multipoint covalent immobilization
Issue Date2013
CitationJournal of Molecular Catalysis B: Enzymatic 98: 8-14 (2013)
AbstractThe production of xylooligosaccharides (XOS) using a packed-bed enzymatic reactor was studied at lab-scale. For this, a xylanase from Aspergillus versicolor was immobilized on different supports. The optimal derivative was xylanase immobilized on glyoxyl-agarose supports. This derivative preserved 85% of its catalytic activity; it was around 700-fold more stable than the soluble enzyme after incubation at 60 C and was able to be reused for at least 10 1 h-cycles retaining full catalytic activity. About 18% of oligosaccharides with prebiotic interest (X2-X6) were produced by the glyoxyl derivative in batch hydrolysis. The production of xylobiose was 2.5-fold higher using the immobilized preparation than with soluble enzyme and small concentrations of xylose (<0.1%) were observed only at the end of the reaction. The derivative was employed on a packed bed reactor, and the continuous operation with no recirculation reached 56% and 70% of the end of reaction with flow rates of 60 mL/h and 12 mL/h, respectively. In continuous operation with recirculation at a flow rate of 60 mL/h, the reaction was completed after four hours. © 2013 Elsevier B.V. All rights reserved.
Identifiersdoi: 10.1016/j.molcatb.2013.09.017
issn: 1381-1177
e-issn: 1873-3158
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(ICP) Artículos
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