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Título: | Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, posttranslational regulation, and compensation of isozyme activities |
Autor: | Rubio Luna, María Carmen CSIC ORCID ; Ramos Escribano, Javier CSIC ORCID ; Webb, K. Judith; Minchin, Frank R.; González, Esther M.; Arrese-Igor, César; Becana Ausejo, Manuel CSIC ORCID | Palabras clave: | Transgene Tissue distribution Superoxide dismutase Plant tissue Plant microorganism interaction Photosynthesis Nodulation Mitochondrion Messenger RNA Legume Genetic line Enzyme activity Chemical compositions Alfalfa Transgenic plant |
Fecha de publicación: | oct-2001 | Editor: | American Phytopathological Society | Citación: | Molecular Plant-Microbe Interactions 14 (10): 1178-1188 (2001) | Resumen: | The composition of antioxidant enzymes, especially superoxide dismutase (SOD), was studied in one nontransgenic and three transgenic lines of nodulated alfalfa plants. Transgenic lines overproduced MnSOD in the mitochondria of nodules and leaves (line 1-10), MnSOD in the chloroplasts (line 4-6), and FeSOD in the chloroplasts (line 10-7). In nodules of line 10-7, the absence of transgene-encoded FeSOD activity was due to a lack of mRNA, whereas in nodules of line 4-6 the absence of transgene-encoded MnSOD activity was due to enzyme inactivation or degradation. Transgenic alfalfa showed a novel compensatory effect in the activities of MnSOD (mitochondrial) and FeSOD (plastidic) in the leaves, which was not caused by changes in the mRNA levels. These findings imply that SOD activity in plant tissues and organelles is regulated, at least partially, at the posttranslational level. All four lines had low CuZnSOD activities and an abundant FeSOD isozyme, especially in nodules, indicating that FeSOD performs important antioxidant functions other than the scavenging of superoxide radicals generated in photosynthesis. This was confirmed by the detection of FeSOD cDNAs and proteins in nodules of other legumes such as cowpea, pea, and soybean. The cDNA encoding alfalfa nodule FeSOD was characterized and the deduced protein found to contain a plastid transit peptide. A comparison of sequences and other properties reveals that there are two types of FeSODs in nodules. | Descripción: | 27 Pags.- 4 Figs.- 2 Tabls. The definitive version, with all 6 Figs., is available at: http://apsjournals.apsnet.org/loi/mpmi | Versión del editor: | http://dx.doi.org/10.1094/MPMI.2001.14.10.1178 | URI: | http://hdl.handle.net/10261/99825 | DOI: | 10.1094/MPMI.2001.14.10.1178 | Identificadores: | doi: 10.1094/MPMI.2001.14.10.1178 issn: 0894-0282 |
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BecanaM_MPMIb_2001.pdf | 1,58 MB | Adobe PDF | Visualizar/Abrir |
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