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Título: | Capillary electrophoresis-mass spectrometry for peptide analysis: Target-based approaches and proteomics/ peptidomics strategies |
Autor: | Simó, Carolina CSIC ORCID ; Cifuentes, Alejandro CSIC ORCID ; Kašička, Václav | Palabras clave: | CE-MS CE Target analysis Proteomics Peptidomics Foodomics Peptides |
Fecha de publicación: | 2013 | Editor: | Humana Press Springer Nature |
Citación: | Capillary Electrophoresis of Biomolecules (Cap.11): 139-151 (2013) | Serie: | Methods in Molecular Biology 984 | Resumen: | In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by the presentation of two different strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligopeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant. The second one is a shotgunlike methodology used for proteomic analysis. Particularly, CE-MS using a time-of- flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics. © Springer Science+Business Media, LLC 2013. | URI: | http://hdl.handle.net/10261/99820 | DOI: | 10.1007/978-1-62703-296-4_11 | Identificadores: | doi: 10.1007/978-1-62703-296-4_11 isbn: 978-1-62703-295-7 |
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