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http://hdl.handle.net/10261/99797
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dc.contributor.author | Díez-Municio, M. | - |
dc.contributor.author | Montilla, Antonia | - |
dc.contributor.author | Moreno, F. Javier | - |
dc.contributor.author | Herrero, Miguel | - |
dc.date.accessioned | 2014-07-14T10:48:42Z | - |
dc.date.available | 2014-07-14T10:48:42Z | - |
dc.date.issued | 2014 | - |
dc.identifier | doi: 10.1039/C3GC42246A | - |
dc.identifier | e-issn: 1463-9270 | - |
dc.identifier | issn: 1463-9262 | - |
dc.identifier.citation | Green Chemistry 16(4): 2219-2226 (2014) | - |
dc.identifier.uri | http://hdl.handle.net/10261/99797 | - |
dc.description.abstract | This work reports the optimization of a cost-effective and scalable process for the enzymatic synthesis of kojibiose (2-O-α-d-glucopyranosyl-α- d-glucose) from readily available and low-cost substrates such as sucrose and lactose. This biotechnological process is based on the dextransucrase-catalysed initial synthesis of a galactosyl-derivative of kojibiose (4-O-β-d- galactopyranosyl-kojibiose) followed by the removal of residual monosaccharides using a Saccharomyces cerevisiae yeast treatment, and a thorough hydrolysis step with Kluyveromyces lactis β-galactosidase. Depending on the final purification stage, i.e. extension of the yeast treatment or the use of preparative liquid chromatography, the purity of the produced kojibiose ranged from 65% to ≥99%, respectively. The moderately high yield achieved (38%, in weight respect to the initial amount of lactose) using this affordable synthesis process could expand the potential applications of kojibiose according to the bioactive properties that have been associated with this disaccharide, so far limited by its low availability. © the Partner Organisations 2014. | - |
dc.description.sponsorship | This work has been financed by projects AGL2011-27884 and Consolider Ingenio 2010 FUN-C-FOOD CSD2007-00063, both from Ministerio de Ciencia e Innovación (MICINN), ALIBIRD 2009/AGR-1469 from Comunidad de Madrid and project POII10-0178-4685 from Junta de Comunidades de Castilla-La Mancha (JCCM) and the European Regional Development Fund (ERDF). M. Díez-Municio is supported by CSIC through the JAE-Pre Programme co-funded by the European Social Fund (ESF). M. Herrero thanks MICINN for his “Ramón y Cajal” contract. | - |
dc.publisher | Royal Society of Chemistry (UK) | - |
dc.relation | S2009/AGR-1469/ALIBIRD | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.title | A sustainable biotechnological process for the efficient synthesis of kojibiose | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1039/C3GC42246A | - |
dc.relation.publisherversion | http://dx.doi.org/10.1039/C3GC42246A | - |
dc.date.updated | 2014-07-14T10:48:42Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Junta de Comunidades de Castilla-La Mancha | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011698 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
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sustainable biotechnologicalrevised version.pdf | 353,06 kB | Adobe PDF | Visualizar/Abrir |
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