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dc.contributor.authorIturbe-Ormaetxe, Iñaki-
dc.contributor.authorHeras, Begoña-
dc.contributor.authorMatamoros Galindo, Manuel Ángel-
dc.contributor.authorRamos Escribano, Javier-
dc.contributor.authorMorán, José F.-
dc.contributor.authorBecana Ausejo, Manuel-
dc.identifierdoi: 10.1034/j.1399-3054.2002.1150107.x-
dc.identifierissn: 0031-9317-
dc.identifier.citationPhysiologia Plantarum 115 (1): 69-73 (2002)-
dc.description16 Pags.- 2 Figs. The definitive version, with all 3 Figs., is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1399-3054-
dc.description.abstractThe thiol tripeptide glutathione (GSH; γGlu-Cys-Gly) is very abundant in legume nodules where it performs multiple functions that are critical for optimal nitrogen fixation. Some legume nodules contain another tripeptide, homoglutathione (hGSH; γGlu-Cys-βAla), in addition to or instead of GSH. We have isolated from a pea (Pisum sativum L.) nodule library a cDNA, GSHS2, that is expressed in nodules but not in leaves. This cDNA was overexpressed in insect cells and its protein product was identified as a highly active and specific hGSH synthetase. The enzyme, the first of this type to be completely purified, is predicted to be a homodimeric cytosolic protein. It shows a specific activity of 3400 nmol hGSH min-1 mg-1 protein with a standard substrate concentration (5 mM β-alanine) and Km values of 1.9 mM for β-alanine and 104 mM for glycine. The specificity constant (Vmax/Km) shows that the pure enzyme is 57.3-fold more specific for β-alanine than for glycine. Southern blot analysis revealed that the gene is present as a single copy in the pea genome and that there are homologous genes in other legumes. We conclude that the synthesis of hGSH in pea nodules is catalysed by a specific hGSH synthetase and not by a GSH synthetase with broad substrate specificity.-
dc.description.sponsorshipM.A.M. was the recipient of a predoctoral fellowship from the Basque Government (Spain). This work was supported by grant no. 2FD97-1101 from the Comisión Interministerial de Ciencia y Tecnología and the European Union and by grant no. PB98-0522 from the Dirección General de Investigación Científica (Spain).-
dc.publisherBlackwell Publishing-
dc.titleCloning and functional characterization of a homoglutathione synthetase from pea nodules-
dc.description.versionPeer Reviewed-
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