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Increases in phosphoenolpyruvate carboxilase activity in iron deficient sugar beet roots: Analysis of spatial localization and post-translational modification

AuthorsAndaluz, Sofía ; López-Millán, Ana Flor ; Peleato Sánchez, María Luisa; Abadía Bayona, Javier ; Abadía Bayona, Anunciación
KeywordsPhosphoenolpyruvate carboxylase
Iron deficiency
Root tips
Sugar beet
Issue DateApr-2002
PublisherKluwer Academic Publishers
CitationPlant and Soil 241 (1): 75-86 (2002)
AbstractRoot tips of Fe-deficient and Fe-sufficient sugar beet plants grown in hydroponics have been used to study the changes in the amount and activity of the cytosolic enzyme phosphoenolpyruvate carboxylase (PEPC, EC Phosphoenolpyruvate carboxylase activity in extracts of the yellow Fe-deficient root tips was, at pH 7.3, 30-fold higher (when expressed on a FW basis) and 7.1-fold higher (when expressed on a protein basis) than that found in the extracts of Fe-sufficient root tips. The amount of phosphoenolpyruvate carboxylase protein determined by immuno-blotting was, on a protein basis, 35-fold larger in the yellow zone of Fe-deficient root tips than in the Fe-sufficient root tips. The inhibition of the phosphoenolpyruvate carboxylase activity by 500 μm malate was 41 and 58% in the extracts Fe-deficient and Fe-sufficient roots. The possibility that post-translational regulation of phosphoenolpyruvate carboxylase may occur mediated through phosphorylation, was studied by immunological detection of phosphoserine residues in root tip extracts.
Publisher version (URL)http://dx.doi.org/10.1023/A:1016000216252
Identifiersdoi: 10.1023/A:1016000216252
issn: 0032-079X
Appears in Collections:(EEAD) Artículos
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