Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/99498
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Discrimination of Xanthomonas campestris pv. campestris races among strains from northwestern Spain by Brassica spp. genotypes and rep-PCR |
Autor: | Lema Márquez, Margarita CSIC ORCID; Cartea González, María Elena CSIC ORCID; Sotelo Pérez, Tamara CSIC; Velasco Pazos, Pablo CSIC ORCID; Soengas Fernández, María del Pilar | Palabras clave: | Genetic variability Molecular characterization Black rot Brassica crops |
Fecha de publicación: | may-2012 | Editor: | Kluwer Academic Publishers | Citación: | European Journal of Plant Pathology 133: 159- 169 (2012) | Resumen: | Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is a severe seedborne disease of Brassica crops around the world. Nine races are recognized, being races 1 and 4 the most aggressive and widespread. The identification of Xcc races affecting Brassica crops in a target area is necessary to establish adequate control measures and breeding strategies. The objectives of this study were to isolate and identify Xcc strains from northwestern Spain by using semi-selective medium and pathogenicity tests, determine the existing races of Xcc in this area by differential series of Brassica spp., and evaluate the use of repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) to differentiate among the nine existing Xcc races. Seventy five isolates recovered from infected fields were identified as Xcc. Race-typing tests determined the presence of the following seven pathogen races: 1, 4, 5, 6, 7, 8 and 9. Race 4 was the most frequent in Brassica oleracea and race 6 in Brassica rapa crops, therefore breeding should be focussed in obtaining resistant varieties to both races. Cluster analysis derived from the combined fingerprints showed four groups, but no clear relationship to race, crop or geographical origin was found. Rep-PCR analysis was found not to be a reliable method to discriminate among Xcc races, therefore race typing of Xcc isolates should be done by using the differential series of Brassica spp. genotypes or another alternative approach. © 2011 KNPV. | Versión del editor: | http://dx.doi.org/10.1007/s10658-011-9929-5 | URI: | http://hdl.handle.net/10261/99498 | DOI: | 10.1007/s10658-011-9929-5 | Identificadores: | doi: 10.1007/s10658-011-9929-5 issn: 0929-1873 e-issn: 1573-8469 |
Aparece en las colecciones: | (MBG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Lema_Discrimination_Xanthomonas...pdf | 211,82 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
34
checked on 22-mar-2024
WEB OF SCIENCETM
Citations
31
checked on 27-feb-2024
Page view(s)
431
checked on 28-mar-2024
Download(s)
439
checked on 28-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.