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Título

Discrimination of Xanthomonas campestris pv. campestris races among strains from northwestern Spain by Brassica spp. genotypes and rep-PCR

AutorLema Márquez, Margarita CSIC ORCID; Cartea González, María Elena CSIC ORCID; Sotelo Pérez, Tamara CSIC; Velasco Pazos, Pablo CSIC ORCID; Soengas Fernández, María del Pilar
Palabras claveGenetic variability
Molecular characterization
Black rot
Brassica crops
Fecha de publicaciónmay-2012
EditorKluwer Academic Publishers
CitaciónEuropean Journal of Plant Pathology 133: 159- 169 (2012)
ResumenBlack rot, caused by Xanthomonas campestris pv. campestris (Xcc) is a severe seedborne disease of Brassica crops around the world. Nine races are recognized, being races 1 and 4 the most aggressive and widespread. The identification of Xcc races affecting Brassica crops in a target area is necessary to establish adequate control measures and breeding strategies. The objectives of this study were to isolate and identify Xcc strains from northwestern Spain by using semi-selective medium and pathogenicity tests, determine the existing races of Xcc in this area by differential series of Brassica spp., and evaluate the use of repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) to differentiate among the nine existing Xcc races. Seventy five isolates recovered from infected fields were identified as Xcc. Race-typing tests determined the presence of the following seven pathogen races: 1, 4, 5, 6, 7, 8 and 9. Race 4 was the most frequent in Brassica oleracea and race 6 in Brassica rapa crops, therefore breeding should be focussed in obtaining resistant varieties to both races. Cluster analysis derived from the combined fingerprints showed four groups, but no clear relationship to race, crop or geographical origin was found. Rep-PCR analysis was found not to be a reliable method to discriminate among Xcc races, therefore race typing of Xcc isolates should be done by using the differential series of Brassica spp. genotypes or another alternative approach. © 2011 KNPV.
Versión del editorhttp://dx.doi.org/10.1007/s10658-011-9929-5
URIhttp://hdl.handle.net/10261/99498
DOI10.1007/s10658-011-9929-5
Identificadoresdoi: 10.1007/s10658-011-9929-5
issn: 0929-1873
e-issn: 1573-8469
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