Strategy for Modular Tagged High-Throughput Amplicon Sequencing Evaluation of Subsampling-Based Normalization Strategies for Tagged High-Throughput Sequencing Data Sets from Gut Microbiomes

Abstract

Several subsampling-based normalization strategies were applied to different high-throughput sequencing data sets originating from human and murine gut environments. Their effects on the data sets’ characteristics and normalization efficiencies, as measured by several -diversity metrics, were compared. For both data sets, subsampling to the median rather than the minimum number appeared to improve the analysis.