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A time-dose model to quantify the antioxidant responses of the oxidative hemolysis inhibition assay (OxHLIA) and its extension to evaluate other hemolytic effectors

AuthorsPrieto, Miguel Ángel ; Vázquez, José Antonio
KeywordsCell based assays
Antioxidant activity
Mathematical moedling
Multivariate analysis
Time-dose response
Issue Date2014
PublisherHindawi Publishing Corporation
CitationBioMed Research International 2014: 632971 (2014)
AbstractThe development of a convenient mathematical application for testing the antioxidant potential of standard and novel therapeutic agents is essential for the research community to perform evaluations in a more precise form. The in vitro oxidative hemolysis inhibition assay (OxHLIA), despite its relevance for in vivo responses, lacks of a proper mathematical model to quantify the responses. In this work, a simple non-linear time-dose tool to test the effectiveness of antioxidant compounds is presented. The model helps to describe accurately the antioxidant response as a function of time and dose. Furthermore, it allows one to perform easily comparisons between different compounds. The model was verified with available experimental data from the bibliography. Its advantages are a simple application, provision of parametric estimates that characterize the response, simplification of the protocol, economization of experimental effort and facilitation of rigorous comparisons among the effects of different compounds and experimental approaches. Finally, other effectors that may obstruct or be of interest for the antioxidant determination are modeled according to similar principles as those defined in the OxHLIA reaction. Thus, the basis of more complex multivariable models is provided. In all experimental data fitted, the calculated parameters were always statistically significant, the equations prove to be consistent and the correlation coefficient of determination was in all cases higher than 0.98
Description27 páginas, 5 tablas, 4 figuras.-- This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Publisher version (URL)http://dx.doi.org/10.1155/2014/632971
Appears in Collections:(IIM) Artículos
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