Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/99136
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Altered characteristics of silica nanoparticles in bovine and human serum: the importance of nanomaterial characterization prior to its toxicological evaluation

AutorIzak-Nau, Emilia; Voetz, Matthias; Eiden, Stefanie; Puntes, Víctor F. CSIC ORCID
Palabras claveSilica nanoparticles
Stability
Protein corona
Nanocharacterization
Fecha de publicación2013
EditorBioMed Central
CitaciónParticle and Fibre Toxicology 10: 56 (2013)
Resumen[Background]: Many toxicological studies on silica nanoparticles (NPs) have been reported, however, the literature often shows various conclusions concerning the same material. This is mainly due to a lack of sufficient NPs characterization as synthesized as well as in operando. Many characteristics of NPs may be affected by the chemistry of their surroundings and the presence of inorganic and biological moieties. Consequently, understanding the behavior of NPs at the time of toxicological assay may play a crucial role in the interpretation of its results. The present study examines changes in properties of differently functionalized fluorescent 50 nm silica NPs in a variety of environments and assesses their ability to absorb proteins from cell culture medium containing either bovine or human serum. [Methods]: The colloidal stability depending on surface functionalization of NPs, their concentration and time of exposure was investigated in water, standard biological buffers, and cell culture media by dynamic light scattering (DLS), zeta potential measurements and transmission electron microscopy (TEM). Interactions of the particles with biological media were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in bovine and human serum, and extracted proteins were assessed using matrix-assisted laser desorption/ionization-time of flight technique (MALDI-TOF). [Results]: It was recognized that all of the studied silica NPs tended to agglomerate after relatively short time in buffers and biological media. The agglomeration depended not only on the NPs functionalization but also on their concentration and the incubation time. Agglomeration was much diminished in a medium containing serum. The protein corona formation depended on time and functionalization of NP, and varied significantly in different types of serum. [Conclusions]: Surface charge, ionic strength and biological molecules alter the properties of silica NPs and potentially affect their biological effects. The NPs surface in bovine serum and in human serum varies significantly, and it changes with incubation time. Consequently, the human serum, rather than the animal serum, should be used while conducting in vitro or in vivo studies concerning humans. Moreover, there is a need to pre-incubate NPs in the serum to control the composition of the bio-nano-composite that would be present in the human body. © 2013 Izak-Nau et al.; licensee BioMed Central Ltd.
DescripciónThis is an open access article distributed under the terms of the Creative Commons Attribution License.
Versión del editorhttp://dx.doi.org/10.1186/1743-8977-10-56
URIhttp://hdl.handle.net/10261/99136
DOI10.1186/1743-8977-10-56
Identificadoresdoi: 10.1186/1743-8977-10-56
issn: 1743-8977
Aparece en las colecciones: (CIN2) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Altered characteristics of silica nanoparticles.pdf1,71 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

28
checked on 29-mar-2024

SCOPUSTM   
Citations

105
checked on 21-mar-2024

WEB OF SCIENCETM
Citations

104
checked on 23-feb-2024

Page view(s)

320
checked on 28-mar-2024

Download(s)

261
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons