English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/99039
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Endosomal maturation by Rab conversion in Aspergillus nidulans is coupled to dynein-mediated basipetal movement

AuthorsAbenza, J. F. ; Galindo, Antonio ; Pinar, Mario ; Pantazopoulou, Areti ; de los Ríos, Vivian; Peñalva, Miguel Ángel
Issue Date15-May-2012
PublisherAmerican Society for Cell Biology
CitationMol Biol Cell. May 15, 2012; 23(10): 1889–1901
AbstractWe exploit the ease with which highly motile early endosomes are distinguished from static late endosomes in order to study Aspergillus nidulans endosomal traffic. RabSRab7 mediates homotypic fusion of late endosomes/vacuoles in a homotypic fusion- and vacuole protein sorting/Vps41–dependent manner. Progression across the endocytic pathway involves endosomal maturation because the end products of the pathway in the absence of RabSRab7 are minivacuoles that are competent in multivesicular body sorting and cargo degradation but retain early endosomal features, such as the ability to undergo long-distance movement and propensity to accumulate in the tip region if dynein function is impaired. Without RabSRab7, early endosomal Rab5s—RabA and RabB—reach minivacuoles, in agreement with the view that Rab7 homologues facilitate the release of Rab5 homologues from endosomes. RabSRab7 is recruited to membranes already at the stage of late endosomes still lacking vacuolar morphology, but the transition between early and late endosomes is sharp, as only in a minor proportion of examples are RabA/RabB and RabSRab7 detectable in the same—frequently the less motile—structures. This early-to-late endosome/vacuole transition is coupled to dynein-dependent movement away from the tip, resembling the periphery-to-center traffic of endosomes accompanying mammalian cell endosomal maturation. Genetic studies establish that endosomal maturation is essential, whereas homotypic vacuolar fusion is not
Description13 p.-11 fig.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.E11-11-0925
URIhttp://hdl.handle.net/10261/99039
DOI10.1091/mbc.E11-11-0925
ISSN1059-1524
E-ISSN1939-4586
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
MBC 2012 Miguel Angel Peñalva.pdf2,6 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.