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dc.contributor.authorAbenza, J. F.-
dc.contributor.authorGalindo, Antonio-
dc.contributor.authorPantazopoulou, Areti-
dc.contributor.authorGil, Concha-
dc.contributor.authorde los Ríos, Vivian-
dc.contributor.authorPeñalva, Miguel Ángel-
dc.identifier.citationMol Biol Cell. Aug 1, 2010; 21(15): 2756–2769es_ES
dc.description14 p.-11 fig.es_ES
dc.description.abstractAspergillus nidulans early endosomes display characteristic long-distance bidirectional motility. Simultaneous dualchannel acquisition showed that the two Rab5 paralogues RabB and RabA colocalize in these early endosomes and also in larger, immotile mature endosomes. However, RabB-GTP is the sole recruiter to endosomes of Vps34 PI3K (phosphatidylinositol-3-kinase) and the phosphatidylinositol-3-phosphate [PI(3)P] effector AnVps19 and rabB , leading to thermosensitivity prevents multivesicular body sorting of endocytic cargo. Thus, RabB is the sole mediator of degradative endosomal identity. Importantly, rabB , unlike rabA , prevents early endosome movement. As affinity experiments and pulldowns showed that RabB-GTP recruits AnVps45, RabB coordinates PI(3)P-dependent endosome-to-vacuole traffic with incoming traffic from the Golgi and with long-distance endosomal motility. However, the finding that Anvps45 ,unlike rabB , severely impairs growth indicates that AnVps45 plays RabB-independent functions. Affinity chromatography showed that the CORVET complex is a RabB and, to a lesser extent, a RabA effector, in agreement with GST pulldown assays of AnVps8. rabB leads to smaller vacuoles, suggesting that it impairs homotypic vacuolar fusion, which would agree with the sequential maturation of endosomal CORVET into HOPS proposed for Saccharomyces cerevisiae.rabB and rabA mutations are synthetically lethal, demonstrating that Rab5-mediated establishment of endosomal identity is essential for A. nidulans.es_ES
dc.description.sponsorshipThis work was supported by Spanish Ministry of Science and Innovation Grants BIO2006-556 and BIO2009-7281 to M.A.P. and by a Comunidad de Madrid Networking Grant SAL/0246/2006 (C.G. and M.A.P.). J.F.A., A.G., and A.P. were supported by Consejo Superior de Investigaciones Cientificas I3P, Formación de Personal Universitario, and Federation of European Biochemical Societies fellowships, respectively.es_ES
dc.publisherAmerican Society for Cell Biologyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleAspergillus RabB Rab5 integrates acquisition of degradative identity with the long distance movement of early endosomeses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-ncsa/ 3.0es_ES
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