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Título

A new technique for screening iron-efficient genotypes in peach rootstocks: Elicitation of root ferric chelate reductase by manipulation of external iron concentrations

AutorGogorcena Aoiz, Yolanda ; Abadía Bayona, Javier ; Abadía Bayona, Anunciación
Palabras clavePrunus persica
Plant nutrition
Prunus
Iron chlorosis
Fecha de publicación2004
EditorTaylor & Francis
CitaciónJournal of Plant Nutrition 27 (10): 1701-1715 (2004)
ResumenThe possibility of using the root ferric chelate reductase enzymatic activity as a tool for iron (Fe) efficient rootstock screening in breeding programs has been investigated. Ferric chelate reductase activities were estimated spectrophotometrically from the formation of the Fe(II)- bathophenantroline complex by exposing the roots of intact plants to nutrient solution containing Fe(III)-EDTA. Four micro-propagated peach rootstocks, GF-677, Adesoto (syn. Puebla de Soto), Barrier and Cadaman, known to differ in tolerance to Fe deficiency under field conditions, were grown in hydroponic cultures containing 90 μM Fe(III)-EDTA. After 9 days, plants were grown without any Fe in the nutrient solution for 4 more days. Then, some plants were re-supplied with 45 and 180 μM of Fe(III)-EDTA for 1, 2, or 3 days. A transient increase in root ferric chelate reductase activity was elicited by Fe resupply in GF-677 and Adesoto, two Fe-efficient genotypes, whereas no induction was found in other two genotypes, Barrier and Cadaman, which are known to be Fe-inefficient. Leaf Fe concentrations after resupply were higher in the Fe-efficient genotypes than in the Fe-inefficient ones. These results indicate that the capacity of the roots to reduce ferric compounds could be used as a screening technique for Fe efficiency, provided it is elicited by manipulation of the external Fe levels. This protocol could be easier to use for commercial breeders looking for Fe-efficient rootstock genotypes.
Versión del editorhttp://dx.doi.org/10.1081/LPLA-200026406
URIhttp://hdl.handle.net/10261/98979
DOI10.1081/LPLA-200026406
Identificadoresdoi: 10.1081/LPLA-200026406
issn: 0190-4167
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