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Título

2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase

AutorFernández-Fueyo, Elena ; Rencoret, Jorge ; Berbís, Manuel Álvaro ; Ruiz-Dueñas, F. J. ; Gutiérrez Suárez, Ana ; Río Andrade, José Carlos del ; Jiménez-Barbero, Jesús ; Hammel, Kenneth E.; Martínez, Ángel T.
Palabras claveLignin
Enzymes
Versatile peroxidase
Veratryl alcohol
2D NMR
Eucalyptus
Fecha de publicación24-jun-2014
EditorCSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)
CitaciónProceedings of the 13th European Workshop on Lignocellulosics and Pulp, pp. 347-350 (2014)
ResumenThe ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals.
URIhttp://hdl.handle.net/10261/98704
ISBN978-84-616-9842-4
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