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A liquid 2,4-D pulse increased shoot and root regeneration from leaf explants of adult Prunus rootstocks

AuthorsPascual Omeñaca, María Lidia ; Marín Velázquez, Juan Antonio
KeywordsAuxin pulse
Genotype effect
Induction treatment
Leaf explant type
Adventitious organ differentiation
Issue Date1-Nov-2005
CitationScientia Horticulturae 106 (4): 582-592 (2005)
AbstractRegeneration of adult plant material is one of the main limitations for successful Prunus rootstock transformation. Results herein show that a liquid pulse (90 min) of 2,4-dichlorophenoxyacetic acid (2,4-D) (1.7 μM), applied to leaf explants, greatly improved shoot regeneration in Marianna 2624 (Prunus cerasifera × munsoniana) and Myrobalan 605 AD (P. cerasifera); and induced roots in Adafuel (Prunus × amigdalo-persica) when placed in regeneration medium. Whole leaves and basal leaf explants of Marianna 2624 regenerated shoots in a higher proportion of explants after the pulse (up to 58.9% in whole leaves) than medium or tip leaf segments, whereas the leaf tip was the explant that showed less regeneration. In the regeneration medium, in the presence of BAP, NAA was more effective than 2,4-D. The application of an auxin pulse is a simple method that could enhance adult plant regeneration in commercial rootstocks.
Identifiersdoi: 10.1016/j.scienta.2005.04.010
issn: 0304-4238
Appears in Collections:(EEAD) Artículos
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