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Título: | Cloning, functional expression and characterization of Mesorhizobium loti arylamine N-acetyltransferases: rhizobial symbiosis supplies leguminous plants with the xenobiotic N-acetylation pathway |
Autor: | Rodrigues-Lima, Fernando; Dairou, Julien; Diaz, Clara L.; Rubio Luna, María Carmen CSIC ORCID ; Sim, Edith; Spaink, Herman P.; Dupret, Jean-Marie | Palabras clave: | Fabaceae Eukaryota Bacteria (microorganisms) Xenobiotic agent Recombinant enzyme Pesticides Isoenzyme Arylamine acetyltransferase Aniline derivatives Lotus corniculatus var. japonicus Mesorhizobium loti Rhizobium Mesorhizobium |
Fecha de publicación: | abr-2006 | Editor: | Blackwell Publishing | Citación: | Molecular Microbiology 60 (2): 505-512 (2006) | Resumen: | Arylamine N-acetyltransferases (NATs) are xenobiotic-metabolizing enzymes involved in the detoxification of numerous aromatic chemicals. The NATdependent N-acetylation pathway has not previously been detected in plants. We demonstrate here the occurrence of the NAT-dependent pathway in leguminous plants, due to symbiosis with Mesorhizobium loti. We cloned two NAT enzymes from M. loti and showed that these two recombinant enzymes catalysed the N-acetylation of several known NAT substrates, including aniline-derived pesticide residues. We also demonstrate the existence of a functional NAT-dependent acetylation pathway in the root nodules of Lotus japonicus inoculated with M. loti. M. loti is the first non-eukaryotic organism shown to express two catalytically active NAT isoforms. This work also provides the first evidence for acquisition of a xenobiotic detoxification pathway by a plant through symbiosis with a soil microbe. | Versión del editor: | http://dx.doi.org/10.1111/j.1365-2958.2006.05114.x | URI: | http://hdl.handle.net/10261/98572 | DOI: | 10.1111/j.1365-2958.2006.05114.x | Identificadores: | doi: 10.1111/j.1365-2958.2006.05114.x issn: 0950-382X |
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