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dc.contributor.authorMalki, Moustafá-
dc.contributor.authorCasado, Santiago-
dc.contributor.authorLópez, María Francisca-
dc.contributor.authorCaillard, Renaud-
dc.contributor.authorPalomares, F. Javier-
dc.contributor.authorVaz-Domínguez, Cristina-
dc.contributor.authorCuesta, Angel-
dc.contributor.authorAmils, Ricardo-
dc.contributor.authorFernández López, Víctor Manuel-
dc.contributor.authorVélez, Marisela-
dc.contributor.authorLacey, Antonio L. de-
dc.contributor.authorOlea, David-
dc.contributor.authorMartín-Gago, José A.-
dc.identifierissn: 1439-4235-
dc.identifier.citationChemphyschem : a European journal of chemical physics and physical chemistry 14(6): 1237- 1244 (2013)-
dc.description.abstractThe biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins. Acidiphilium biofilms are grown on graphitic flakes and are characterized by different physicochemical techniques. Combined fluorescence-AFM microscopy is applied to study the biofilm formation over time and to detect nanosized particles that are, in turn, analyzed by X-ray photoelectron spectroscopy and attenuated total reflection surface-enhanced infrared absorption spectroscopy. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.-
dc.description.sponsorshipSpanish Ministerio de Ciencia e Innovación (projects CTQ2009-12649, MAT2011-26534, MAT2010-18432); Fundación Ramón Areces (project 20100272)-
dc.publisherJohn Wiley & Sons-
dc.titlePhysicochemical characterization of Acidiphilium sp. biofilms-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderFundación Ramón Areces-
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