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Title

Acetylation of vitamin E by Candida antarctica lipase B immobilized on different carriers

AuthorsTorres, Pamela ; Reyes-Duarte, Dolores; López-Cortés, Nieves ; Ferrer, Manuel ; Ballesteros Olmo, Antonio ; Plou Gasca, Francisco José
KeywordsIMMOBILIZATION
TOCOPHEROLS
ANTIOXIDANTS
TRANSESTERIFICATION
STABILIZATION
Issue Date23-Jan-2009
SeriesProcess Biochemistry 42, 145-153 (2008)
AbstractWe describe for the first time the enzymatic acylation of the phenolic group of tocopherols (vitamin E) by transesterification with vinyl acetate in 2-methyl-2-butanol (2M2B). Out of 15 hydrolases screened, only the lipase B from Candida antarctica (Novozym 435) catalyzed the acylation. The acetylation of -tocopherol was faster than that of -tocopherol, probably due to its lower methylation degree. A series of experiments using (R)-Trolox and p-cresol as competitive acceptors of tocopherols showed that reaction rate notably diminished when increasing acceptor size. To maximize the potential of this reaction, three immobilization carriers for C. antarctica lipase B were studied: the ion-exchange resin Lewatit (the support in Novozym 435), a biodegradable polymer (Purasorb) and polypropylene (Accurel EP100). The acetylation of -tocopherol was faster with the enzyme immobilized in polypropylene, which was correlated with its higher porosity. A mixture hexane/2M2B 90:10 (v/v) was found to be the optimum medium composition, as it represents a compromise between substrates solubility and biocatalyst efficiency. The acylation process was no enantioselective, probably due to the fact that the chiral centers are separated from the phenolic group by a minimum of six bonds.
Publisher version (URL)http://dx.doi.org/10.1016/j.procbio.2007.11.008
URIhttp://hdl.handle.net/10261/9822
DOI10.1016/j.procbio.2007.11.008
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