Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/9805
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Título : Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin
Autor : Canales García-Menocal, Mario, Naranjo, María Victoria, Almazán, Consuelo, Molina, Ricardo, Tsuruta, Suzana A., Szabó, Matias P. J., Manzano Román, Raúl, Pérez de Lastra, José Manuel, Kocan, Katherine M., Jiménez, María Isabel, Lucientes Curdi, Javier, Villar, Margarita, Fuente García, José de la
Fecha de publicación : 20-Feb-2009
Editor: Springer
Citación : Parasitology Research 105(1): 97-111 (2009)
Resumen: The control of arthropod vectors of pathogens that affect human and animal health is important for the eradication of vector-borne diseases. The ortholog of the tick-protective antigen, subolesin, was identified in Aedes albopictus and found to have conserved epitopes in ticks and mosquitoes. RNA interference with the tick and mosquito double-stranded RNA in three tick species resulted in significant gene knockdown and decreased tick weight and/or survival. Feeding Anopheles atroparvus, Aedes caspius, and Culex pipiens female mosquitoes on an A. albopictus subolesin hyperimmune serum resulted in 11 ± 5% to 29 ± 6% survival inhibition when compared to controls fed on preimmune serum. Feeding sand flies, Phlebotomus perniciosus, on antimosquito subolesin ortholog protein antibodies inhibited female survival and the number of larvae and adults obtained after hatching by 28 ± 22% and 16 ± 3%, respectively, when compared to controls. Vaccination with tick and mosquito subolesin ortholog proteins significantly reduced Ixodes scapularis tick infestation and weight in a similar way. However, vaccination with the recombinant mosquito subolesin ortholog antigen did not protect against Amblyomma americanum and Rhipicephalus sanguineus tick infestations. Collectively, these preliminary results provided the first evidence that development of vaccines may be possible for control of multiple arthropod vectors using subolesin orthologs but suggested that multiple antigens may be required to produce an effective vaccine.
Descripción : 15 pages, 5 figures, 6 tables.-- PMID: 19229557 [PubMed].-- Printed version published Jul 2009.
Versión del editor: http://dx.doi.org/10.1007/s00436-009-1368-2
URI : http://hdl.handle.net/10261/9805
ISSN: 0932-0113 (Print)
1432-1955 (Online)
DOI: 10.1007/s00436-009-1368-2
Citación : Parasitology Research 105(1): 97-111 (2009)
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