English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/96912
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

An intermolecular disulfide-based light switch for chloroplast psbD gene expression in Chlamydomonas reinhardtii

AuthorsSchwarz, Christian; Bohne, Alexandra; Cejudo, Francisco Javier ; Nickelsen, Jörg
KeywordsChloroplast gene expression: Nac2, redox control
RNP
Chlamydomonas
Issue Date2012
PublisherBlackwell Publishing
CitationPlant Journal 72: 378- 389 (2012)
AbstractExpression of the chloroplast psbD gene encoding the D2 protein of the photosystem II reaction center is regulated by light. In the green alga Chlamydomonas reinhardtii, D2 synthesis requires a high-molecular-weight complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Based on size exclusion chromatography analyses, we provide evidence that light control of D2 synthesis depends on dynamic formation of the Nac2/RBP40 complex. Furthermore, 2D redox SDS-PAGE assays suggest an intermolecular disulfide bridge between Nac2 and Cys11 of RBP40 as the putative molecular basis for attachment of RBP40 to the complex in light-grown cells. This covalent link is reduced in the dark, most likely via NADPH-dependent thioredoxin reductase C, supporting the idea of a direct relationship between chloroplast gene expression and chloroplast carbon metabolism during dark adaption of algal cells. © 2012 Blackwell Publishing Ltd.
URIhttp://hdl.handle.net/10261/96912
DOI10.1111/j.1365-313X.2012.05083.x
Identifiersdoi: 10.1111/j.1365-313X.2012.05083.x
issn: 0960-7412
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
TPJ-ms.pdf2,82 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.