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Title

Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers

AuthorsKunamneni, Adinarayana CSIC ORCID; Ghazi, Iraj CSIC; Camarero, Susana CSIC ORCID ; Ballesteros Olmo, Antonio CSIC ORCID; Plou Gasca, Francisco José CSIC ORCID ; Alcalde Galeote, Miguel CSIC ORCID
KeywordsCovalent immobilization
Epoxy Carriers
Acrylic polymers
Synthetic dyes
Biocatalysis
Biotransformations
Issue DateFeb-2008
PublisherElsevier
CitationProcess Biochemistry 43(2): 169-178 (2008)
AbstractThe Myceliophthora thermophila laccase was covalently immobilized on polymethacrylate-based polymers (Sepabeads EC-EP3 and Dilbeads NK) activated with epoxy groups. The enzyme immobilized on Sepabeads EC-EP3 exhibited notable activity (203 U/g) along with remarkably improved stability towards pH, temperature and storage time, but no increased resistance to organic solvents. In addition, the immobilized laccase also showed good operational stability, maintaining 84% of its initial activity after 17 cycles of oxidation of ABTS. The immobilized biocatalyst was applied to the decolorization of six synthetic dyes. Immobilized laccase retained 41% activity in the decolorization of Methyl Green in a fixed-bed reactor after five cycles. The features of these biocatalysts are very attractive for their application on the decolorization of dyes in the textile industry in batch and continuous fixed-bed bioreactors. To our knowledge, this is the first report on immobilization of laccase on Sepabeads carriers and its efficient dyes decolorization.
Publisher version (URL)http://doi.org/10.1016/j.procbio.2007.11.009
URIhttp://hdl.handle.net/10261/9672
DOI10.1016/j.procbio.2007.11.009
ISSN1359-5113
E-ISSN1873-3298
Appears in Collections:(ICP) Artículos
(CIB) Artículos




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