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dc.contributor.authorCistué Sola, Luis-
dc.contributor.authorRomagosa, Ignacio-
dc.contributor.authorBatlle, F.-
dc.contributor.authorEchávarri Razquín, Begoña-
dc.identifierdoi: 10.1007/s00299-009-0690-6-
dc.identifierissn: 0721-7714-
dc.identifiere-issn: 1432-203X-
dc.identifier.citationPlant Cell Reports 28 (5): 727-735 (2009)-
dc.description27 Pags.- 2 Tabls.- 2 Figs. The definitive version is available at: http://link.springer.com/journal/299-
dc.description.abstractThe objective of this study was to produce durum wheat doubled haploid (DH) plants through the induction of microspore embryogenesis. The microspore culture technique was improved to maximize production of green plants per spike using three commercial cultivars. Studies on factors such as induction media composition, induction media support and the stage and growth of donor plants were carried out in order to develop an efficient protocol to regenerate green and fertile DH plants. Microspores were plated on a C17 induction culture medium with ovary co-culture and a supplement of glutathione plus glutamine; 300 g/l Ficoll Type-400 was incorporated to the induction medium support. Donor plants were fertilized with a combination of macro and microelements. With the cultivars 'Ciccio' and 'Claudio' an average of 36.5 and 148.5 fertile plants were produced, respectively, from 1,000 anthers inoculated. This technique was then used to produce fertile DH plants of potential agronomic interest from a collection of ten F1 crosses involving cultivars of high breeding value. From these crosses 849 green plants were obtained and seed was harvested from 702 plants indicating that 83% of green plants were fertile and therefore were spontaneously DHs. No aneuploid plant was obtained. The 702 plants yielded enough seeds to be field tested. One of the DH lines obtained by microspore embryogenesis, named 'Lanuza', has been sent to the Spanish Plant Variety Office for Registration by the Batlle Seed Company. This protocol can be used instead of the labor-intensive inter-generic crossing with maize as an economically feasible method to obtain DHs for most crosses involving the durum wheat cultivars grown in Spain.-
dc.description.sponsorshipThe research was supported by Projects AGL2005-07195-C02 and AGL2008-05541-C02 from the Spanish Ministry of Science and Technology. We also want to thank Batlle Seeds Company S.A. and Agromonegros Seeds Company S.A. for their support of this research.-
dc.subjectMicrospore culture-
dc.subjectTriticum turgidum ssp. durum-
dc.titleImprovements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture-
dc.description.versionPeer Reviewed-
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