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Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentation

AuthorsRodríguez-Colinas, Bárbara ; Kolida, Sofia; Baran, Magdalena; Ballesteros Olmo, Antonio ; Rastall, Robert A.; Plou Gasca, Francisco José
KeywordsBatch culture system
Short-chain fatty acids
Issue Date2013
CitationApplied Microbiology and Biotechnology, Volume 97, Issue 13, pp 5743-5752 (2013)
AbstractThe in vitro fermentation properties of several purified galacto-oligosaccharides (GOS), specifically the trisaccharides 4’-galactosyl-lactose and 6’-galactosyl-lactose, and a mixture of the disaccharides 6-galactobiose and allolactose, was carried out. The bifidogenic effect of GOS at 1% (w/v) was studied in a pH-controlled batch culture fermentation system inoculated with healthy adult human faeces. Results were compared with those obtained with a commercial GOS mixture (Bimuno-GOS). Changes in bacterial populations measured through fluorescence in situ hybridization (FISH) and short-chain fatty acid (SCFA) production were determined. Bifidobacteria increased after 10 h fermentation for all the GOS substrates, but the changes were only statistically significant (P < 0.05) for the mixture of disaccharides and Bimuno-GOS. Acetic acid, whose formation is consistent with bifidobacteria metabolism, was the major SCFA synthesized. The acetate concentration at 10 h was similar with all the substrates (45-50 mM) and significantly higher than the observed for formic, propionic and butyric acids. All the purified GOS could be considered bifidogenic under the assayed conditions, displaying a selectivity index (SI) in the range 2.1-3.0, which was slightly lower than the determined for the commercial mixture Bimuno-GOS
Publisher version (URL)http://dx.doi.org/10.1007/s00253-013-4892-z
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