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dc.contributor.authorAyuso-Fernández, Iván-
dc.contributor.authorGalmés, Miquel A.-
dc.contributor.authorBastida, Agatha-
dc.contributor.authorGarcía-Junceda, Eduardo-
dc.date.accessioned2014-04-24T08:43:36Z-
dc.date.available2014-04-24T08:43:36Z-
dc.date.issued2014-
dc.identifier.citationChemCatChem 6 (4) : 1059-1065 (2014)es_ES
dc.identifier.issn1867-3880-
dc.identifier.urihttp://hdl.handle.net/10261/95814-
dc.description.abstractSulfation is an important molecular modification that regulates essential cellular processes and is also implicated in numerous pathological processes. The enzymes responsible for this reaction in living organisms are sulfotransferases. The gene Hoch_5094 from Haliangium ochraceum is annotated as a putative sulfotransferase. The arylsulfotransferase codified by this gene (HocAST) was expressed heterologously in E. coli and showed aryl sulfotransferase activity. Circular dichroism analysis of HocAST showed a main α/β secondary structure that agrees with the overall structure of other cytosolic sulfotransferases. Interestingly, HocAST was able to use both p-nitrophenyl sulfate and 3′-phosphoadenosine-5′-phosphosulfate (PAPS) as sulfuryl donors contrary to that of aryl sulfate sulfotransferase, which cannot use PAPS as a donor. Regarding the specificity towards the acceptor, HocAST has shown quite a wide scope and was able to accept several mono- and dihydroxylated phenols and other phosphorylated compounds as substrates.es_ES
dc.description.sponsorshipSpanish Ministerio de Ciencia e Innovación (Grants PI11/01436 and CTQ2010-19073/BQU), Comunidad de Madrid (Grant S2009/PPQ-1752) and MAPFRE Foundation for financial support. We thank Dr. R. Wohlgemuth for the gift of PAPS and PAP.es_ES
dc.language.isoenges_ES
dc.publisherWiley-VCHes_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectbiocatalysises_ES
dc.subjectbioorganic chemistryes_ES
dc.subjectbiotransformationses_ES
dc.subjectenzymeses_ES
dc.subjectsulfationes_ES
dc.titleAryl Sulfotransferase from Haliangium ochraceum: A Versatile Tool for the Sulfation of Small Moleculeses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/cctc.201300853-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/cctc.201300853es_ES
dc.identifier.e-issn1867-3899-
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