Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/95633
COMPARTIR / EXPORTAR:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses

AutorSánchez-Sevilla, José F; Cruz-Rus, Eduardo; Valpuesta, Victoriano; Botella, Miguel Angel; Amaya, Iraida
Fecha de publicación17-abr-2014
EditorBioMed Central
CitaciónBMC Genomics.15(1):218 (2014)
ResumenAbstract Background Understanding the basis for volatile organic compound (VOC) biosynthesis and regulation is of great importance for the genetic improvement of fruit flavor. Lactones constitute an essential group of fatty acid-derived VOCs conferring peach-like aroma to a number of fruits including peach, plum, pineapple and strawberry. Early studies on lactone biosynthesis suggest that several enzymatic pathways could be responsible for the diversity of lactones, but detailed information on them remained elusive. In this study, we have integrated genetic mapping and genome-wide transcriptome analysis to investigate the molecular basis of natural variation in γ-decalactone content in strawberry fruit. Results As a result, the fatty acid desaturase FaFAD1 was identified as the gene underlying the locus at LGIII-2 that controls γ-decalactone production in ripening fruit. The FaFAD1 gene is specifically expressed in ripe fruits and its expression fully correlates with the presence of γ-decalactone in all 95 individuals of the mapping population. In addition, we show that the level of expression of FaFAH1, with similarity to cytochrome p450 hydroxylases, significantly correlates with the content of γ-decalactone in the mapping population. The analysis of expression quantitative trait loci (eQTL) suggests that the product of this gene also has a regulatory role in the biosynthetic pathway of lactones. Conclusions Altogether, this study provides mechanistic information of how the production of γ-decalactone is naturally controlled in strawberry, and proposes enzymatic activities necessary for the formation of this VOC in plants.
URIhttp://hdl.handle.net/10261/95633
Identificadoreshttp://dx.doi.org/10.1186/1471-2164-15-218
Aparece en las colecciones: (IHSM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
1471-2164-15-218.xml125,76 kBXMLVisualizar/Abrir
1471-2164-15-218.pdf2,89 MBAdobe PDFVista previa
Visualizar/Abrir
1471-2164-15-218-S2.XLSX251,06 kBMicrosoft Excel XMLVisualizar/Abrir
1471-2164-15-218-S1.PDF1,39 MBAdobe PDFVista previa
Visualizar/Abrir
1471-2164-15-218-S3.XLSX6,24 MBMicrosoft Excel XMLVisualizar/Abrir
Mostrar el registro completo

CORE Recommender

Page view(s)

296
checked on 29-mar-2024

Download(s)

931
checked on 29-mar-2024

Google ScholarTM

Check


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.