In vitro rumen methane output of grasses and grass silages differing in fermentation characteristics using the gas-production technique (GPT)

Abstract

The chemical composition of silage consumed by cattle can influence the subsequent rumen microbial fermentation patterns and methane (CH4) emissions. The objectives of this study were to (i) evaluate the effect of ensilage on the in vitro rumen methane output of perennial ryegrass and (ii) relate the silage fermentation characteristics of grass silages with in vitro rumen methanogenesis. Three pre-harvest herbage-conditioning treatments and seven silage-additive treatments were used in a laboratory-scale silo experiment to produce a diversity of silage fermentation characteristics. Ensilage reduced (P<0·01) the in vitro rumen CH4 output (mLCH4g-1 dry matter (DM) disappeared). This reflected differences in the direction of rumen fermentation (lower acetic (P<0·05) and higher propionic (P<0·001) acid proportions in volatile fatty acids) rather than major changes in the extent of in vitro rumen fermentation (i.e. mmolVFAg-1 DM). The magnitude of the decrease in CH4 output (mLg-1 DM incubated) owing to ensilage increased as the extent of silage fermentation, and in particular the lactic acid concentration, increased. In contrast, among silages with relatively similar extents of silage fermentation (i.e. total fermentation products), an increase in the proportion of lactic acid in silage fermentation products led to a more extensive in vitro rumen fermentation and thus to a greater CH4 output (mLg-1 DM). © 2012 Blackwell Publishing Ltd.