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Title

Lactate biosensor based on a bionanocomposite composed of titanium oxide nanoparticles, photocatalytically reduced graphene, and lactate oxidase

AuthorsCasero, Elena; Alonso, Concepción; Petit-Domínguez, M. Dolores; Vázquez, Luis ; Parra-Alfambra, A. M.; Merino-Mateo, Pablo ; Álvarez-García, Susana; Andrés, Alicia de ; Lorenzo, Encarnación
KeywordsBionanocomposite
Photocatalitically reduced graphene
Titanium dioxide NPs
Biosensors platforms
Lactate oxidase
Issue Date2014
PublisherSpringer
CitationMicrochimica Acta 181(1-2): 79-87 (2014)
AbstractWe have developed a lactate biosensor based on a bionanocomposite (BNC) composed of titanium dioxide nanoparticles (TiO2-NPs), photocatalytically reduced graphene, and lactate oxidase. Graphene oxide was photochemically reduced (without using any chemical reagents) in the presence of TiO2-NPs to give graphene nanosheets that were characterized by atomic force microscopy, Raman and X-ray photoelectron spectroscopy. The results show the nanosheets to possess few oxygen functionalities only and to be decorated with TiO2-NPs. These nanosheets typically are at least 1 μm long and have a thickness of 4.2 nm. A BNC was obtained by mixing lactate oxidase with the nanosheets and immobilized on the surface of a glassy carbon electrode. The resulting biosensor was applied to the determination of lactate. Compared to a sensor without TiO2-NPs, the sensor exhibits higher sensitivity (6.0 μA mM-1), a better detection limit (0.6 μM), a wider linear response (2.0 μM to 0.40 mM), and better reproducibility (3.2 %). [Figure not available: see fulltext.] © 2013 Springer-Verlag Wien.
DescriptionEl pdf del artículo es la versión post-print.-- et al.
Publisher version (URL)http://dx.doi.org/10.1007/s00604-013-1070-z
URIhttp://hdl.handle.net/10261/94643
DOI10.1007/s00604-013-1070-z
Identifiersdoi: 10.1007/s00604-013-1070-z
issn: 0026-3672
e-issn: 1436-5073
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(ICMM) Artículos
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