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Closed Access item 3D structure of the influenza virus polymerase complex: Localization of subunit domains

Authors:Area, Estela
Martín-Benito, Jaime
Gastaminza, Pablo
Torreira, Eva
Valpuesta, José M.
Carrascosa, José L.
Ortín, Juan
Keywords:Influenza A virus, Polymerase complex, Ribonucleoproteins (RNPs), Polymerase subunits, Structural model
Issue Date:6-Jan-2004
Publisher:National Academy of Sciences (U.S.)
Citation:Proc. Natl. Acad. Sci. USA 101(1): 308-313 (2004)
Abstract:The 3D structure of the influenza virus polymerase complex was determined by electron microscopy and image processing of recombinant ribonucleoproteins (RNPs). The RNPs were generated by in vivo amplification using cDNAs of the three polymerase subunits, the nucleoprotein, and a model virus-associated RNA containing 248 nt. The polymerase structure obtained is very compact, with no apparent boundaries among subunits. The position of specific regions of the PB1, PB2, and PA subunits was determined by 3D reconstruction of either RNP–mAb complexes or tagged RNPs. This structural model is available for the polymerase of a negative-stranded RNA virus and provides a general delineation of the complex and its interaction with the template-associated nucleoprotein monomers in the RNP.
Description:6 pages, 6 figures.-- PMID: 14691253 [PubMed].-- PMCID: PMC314181.-- Published online before print Dec 22, 2003.
Full-text article available Open Access via PubMed Central: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=14691253
Publisher version (URL):http://dx.doi.org/10.1073/pnas.0307127101
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