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Título: | The distribution of fitness effects caused by single-nucleotide substitutions in an RNA virus |
Autor: | Sanjuán, Rafael CSIC ORCID; Moya-Simarro, Andrés; Elena, Santiago F. CSIC ORCID | Palabras clave: | Mutational fitness effects RNA viral genomes Single-nucleotide substitutions Evolutionary biology |
Fecha de publicación: | 24-may-2004 | Editor: | National Academy of Sciences (U.S.) | Citación: | Proc. Natl. Acad. Sci. USA 101(22): 8396-8401 (2004) | Resumen: | Little is known about the mutational fitness effects associated with single-nucleotide substitutions on RNA viral genomes. Here, we used site-directed mutagenesis to create 91 single mutant clones of vesicular stomatitis virus derived from a common ancestral cDNA and performed competition experiments to measure the relative fitness of each mutant. The distribution of nonlethal deleterious effects was highly skewed and had a long, flat tail. As expected, fitness effects depended on whether mutations were chosen at random or reproduced previously described ones. The effect of random deleterious mutations was well described by a log-normal distribution, with -19% reduction of average fitness; the effects distribution of preobserved deleterious mutations was better explained by a β model. The fit of both models was improved when combined with a uniform distribution. Up to 40% of random mutations were lethal. The proportion of beneficial mutations was unexpectedly high. Beneficial effects followed a γ distribution, with expected fitness increases of 1% for random mutations and 5% for preobserved mutations. | Descripción: | 6 pages, 3 figures.-- PMID: 15159545 [PubMed].-- PMCID: PMC420405.-- Supporting information (Table 3: Relevant information about each single-nucleotide substation mutant created) available at: http://www.pnas.org/content/101/22/8396/suppl/DC1 Printed version published on Jun 1, 2004. Full-text paper available Open Access via PubMed Central: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=15159545 |
Versión del editor: | http://dx.doi.org/10.1073/pnas.0400146101 | URI: | http://hdl.handle.net/10261/9417 | DOI: | 10.1073/pnas.0400146101 | ISSN: | 0027-8424 |
Aparece en las colecciones: | (IBMCP) Artículos |
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