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http://hdl.handle.net/10261/9398
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Aurtenetxe, Olaia | es_ES |
dc.contributor.author | Barral, Marta | es_ES |
dc.contributor.author | Vicente, Joaquín | es_ES |
dc.contributor.author | Fuente, José de la | es_ES |
dc.contributor.author | Gortázar, Christian | es_ES |
dc.contributor.author | Juste, Ramón A. | es_ES |
dc.date.accessioned | 2008-12-30T08:21:54Z | - |
dc.date.available | 2008-12-30T08:21:54Z | - |
dc.date.issued | 2008-11-01 | - |
dc.identifier.citation | BMC Veterinary Research 4: 43 (2008) | es_ES |
dc.identifier.issn | 1746-6148 | - |
dc.identifier.uri | http://hdl.handle.net/10261/9398 | - |
dc.description.abstract | [Background] Bovine tuberculosis (bTB) remains a significant problem in some parts of Spain largely because of contacts between cattle and wildlife reservoirs in extensive grazing systems. European Wild boar (Sus scrofa) is one of the species involved in the transmission of the disease to other species. Fast and simple detection methods would be critical for assessing infection prevalence, study the mechanisms of pathogen transmission and monitoring the effects of TB control measures. | es_ES |
dc.description.abstract | [Results] An enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Mycobacterium bovis in wild boar serum was developed and validated on 185 sera from TB positive and negative wild boar. Based on antigen inoculation of captive animals as well as tuberculosis compatible lesions, culture results and molecular analysis of hunted individuals, animals were allocated into two groups: tuberculosis positive group and tuberculosis negative group. After optimization of the positive to negative ratio using different combinations of serum dilutions and conjugate concentrations, the test yielded a sensitivity of 72.60% and a specificity of 96.43% for the best cut-off. | es_ES |
dc.description.abstract | [Conclusion] Although some negative group animals showed an ELISA positive reaction (< 3%), this assay showed a high potential for accurate diagnosis of TB in wild boar, as its large dynamic range supported a good discriminatory power and a satisfactory balance between sensitivity and specificity. | es_ES |
dc.description.sponsorship | This work was supported by projects INIARTA03-074-C2 and "Control of tuberculosis in Wildlife" of Santander-Fundación Marcelino Botín (to C. G. and J. F.); Department of Agriculture, Fisheries and Food of the Basque Government; CICYT-MEC research Grant AGL2005-07401, and FEDER (Spain). This work was made in part thanks to the agreements with OAPN and SDGSA (MMA-MRM); MAPA; Fundación Cándido de Iturriaga y María de Dañobeitia and Diputaciones Forales de Vizcaya, Guipúzcoa y Álava. | es_ES |
dc.format | application/pdf | es_ES |
dc.format.extent | 333870 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Bovine tuberculosis | es_ES |
dc.subject | Simple detection methods | es_ES |
dc.subject | European Wild boar | es_ES |
dc.subject | Sus scrofa | es_ES |
dc.subject | Mycobacterium bovis | es_ES |
dc.subject | Antibodies | es_ES |
dc.subject | Enzyme-linked immunosorbent assay (ELISA) | es_ES |
dc.title | Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovis in european wild boar | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1186/1746-6148-4-43 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1186/1746-6148-4-43 | es_ES |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | es_ES |
dc.contributor.funder | Eusko Jaurlaritza | es_ES |
dc.contributor.funder | Organismo Autónomo Parques Nacionales (España) | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003086 | es_ES |
dc.identifier.pmid | 18976491 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.openairetype | artículo | - |
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Development_validation_immunosorbent_assay.pdf | 326,04 kB | Adobe PDF | Visualizar/Abrir |
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