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Optimization of pressurized liquid extraction of inositols from pine nuts (Pinus pinea L.)

AuthorsRuiz Aceituno, Laura ; Rodríguez-Sánchez, Sonia ; Sanz Perucha, Jesús ; Sanz, M. Luz ; Ramos, Lourdes
KeywordsPine nuts
Pressurized liquid extraction
Saccharomyces cerevisiae
Issue Date2014
CitationFood Chemistry 153: 450- 456 (2014)
AbstractPressurized liquid extraction (PLE) has been used for the first time to extract bioactive inositols from pine nuts. The influence of extraction time, temperature and cycles of extraction in the yield and composition of the extract was studied. A quadratic lineal model using multiple linear regression in the stepwise mode was used to evaluate possible trends in the process. Under optimised PLE conditions (50 C, 18 min, 3 cycles of 1.5 mL water each one) at 10 MPa, a noticeable reduction in extraction time and solvent volume, compared with solid-liquid extraction (SLE; room temperature, 2 h, 2 cycles of 5 mL water each one) was achieved; 5.7 mg/g inositols were extracted by PLE, whereas yields of only 3.7 mg/g were obtained by SLE. Subsequent incubation of PLE extracts with Saccharomyces cerevisiae (37 C, 5 h) allowed the removal of other co-extracted low molecular weight carbohydrates which may interfere in the bioactivity of inositols. © 2014 Elsevier Ltd. All rights reserved.
Identifiersdoi: 10.1016/j.foodchem.2013.12.079
issn: 0308-8146
Appears in Collections:(IQOG) Artículos
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