English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/92032
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Morphologic, cytometric and functional characterization of the common octopus (Octopus vulgaris) hemocytes

AuthorsCastellanos-Martínez, Sheila ; Prado-Álvarez, María ; Lobo da Cunha, Alexandre; Azevedo, Carlos; Gestal, C.
KeywordsOctopus vulgaris
Hemocytes
Flow cytometry
Functional characterization
Immune defense
Issue Date2014
PublisherElsevier
CitationDevelopmental and Comparative Immunology 44(1): 50-58 (2014)
AbstractThe hemocytes of Octopus vulgaris were morphologically and functionally characterized. Light and electron microscopy (TEM and SEM), and flow cytometry analyses revealed the existence of two hemocyte populations. Large granulocytes showed U-shaped nucleus, a mean of 11.6 μm ± 1.2 in diameter with basophilic granules, polysaccharide and lysosomic deposits in the cytoplasm. Small granulocytes measured a mean of 8.1 μm ± 0.7 in diameter, and have a round nucleus occupying almost the entire cell and few or not granules in the cytoplasm. Flow cytometry analysis showed that large granulocytes are the principal cells that develop phagocytosis of latex beads (rising up to 56%) and ROS after zymosan stimulation. Zymosan induced the highest production of both ROS and NO. This study is the first tread towards understanding the O. vulgaris immune system by applying new tools to provide a most comprehensive morpho-functional study of their hemocytes.
Description9 páginas, 7 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.dci.2013.11.013
URIhttp://hdl.handle.net/10261/92032
DOI10.1016/j.dci.2013.11.013
ISSN0145-305X
E-ISSN1879-0089
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.