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dc.contributor.authorSobrino-Plata, Juan-
dc.contributor.authorCarrasco-Gil, Sandra-
dc.contributor.authorAbadía Bayona, Javier-
dc.contributor.authorEscobar, Carolina-
dc.contributor.authorÁlvarez-Fernández, Ana-
dc.contributor.authorHernández, Luis E.-
dc.identifier.citationSobrino-Plata J, Carrasco-Gil S, Abadía J, Escobar C, Álvarez-Fernández A, Hernández LE. The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis. Metallomics 6 (2): 356-366 (2014)es_ES
dc.description32 pag., 6 fig., 1 tabl., suppl. mat. Available online 2 January 2014. The definitive version is available at: http://pubs.rsc.org/en/content/articlelanding/2013/mt/c3mt00329aes_ES
dc.description.abstractRecent research efforts have highlighted the importance of glutathione (GSH) as a key antioxidant metabolite for metal tolerance in plants. Little is known about the mechanisms involved in stress due to mercury (Hg), one of the most hazardous metals to the environment and human health. To understand the implication of GSH metabolism in Hg tolerance, we used two γ-glutamylcysteine synthetase (γECS) Arabidopsis thaliana allele mutants (rax1-1 and cad2-1) and a phytochelatin synthase (PCS) mutant (cad1-3). The leaves of these mutants and of wild type (Col-0) were infiltrated with a solution containing Cd or Hg (0, 3 and 30 µM) and incubated for 24 and 48 h. The formation of phytochelatins (PCs) in the leaf extracts was followed by two different HPLC-based methods and occurred in Col-0, cad2-1 and rax1-1 plants exposed to Cd, whereas in the Hg treatments, PCs accumulated mainly in Col-0 and rax1-1, where Hg-PC complexes were also detected. ASA and GSH/GSSG levels increased under moderate metal stress conditions, accompanied by increased GSH reductase (GR) activity and expression. However, higher metal doses led to a decrease in the analysed parameters, and stronger toxic effects appeared with 30 µM Hg. The GSH concentration was significantly higher in rax1-1 (70% of Col-0) than in cad2-1 (40% of Col-0). The leaves of rax1-1 were less sensitive than cad2-1, in accordance with the greater expression of γECS in rax1-1. Our results underline the existence of a minimal GSH concentration threshold needed to minimise the toxic effects exerted by Hg.es_ES
dc.description.sponsorshipThis work was funded by the Ministry of Economy and Competitivity (Projects PROBIOMET AGL2010-15151 and AGL2010-16515), Junta Comunidades Castilla La Mancha (FITOALMA2, POII10-007-6458), Comunidad de Madrid (EIADES consortium S2009/AMB 1478) and the Aragón Government (Group A03)es_ES
dc.publisherRoyal Society of Chemistry (UK)es_ES
dc.titleThe role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsises_ES
dc.description.peerreviewedPeer reviewedes_ES
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