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Title

Analysis of the efficiency of the rabbit whey acidic protein gene 5′ flanking region in controlling the expression of homologous and heterologous linked genes

AuthorsDevinoy, Eve; Montoliu, Lluís CSIC ORCID ; Baranyi, Mária; Thépot, Dominique; Hiripi, László; Fontaine, Marie-Louise; Bodrogi, Lilla; Bosze, Zsuzsanna
KeywordsWhey acidic protein
Regulatory regions
Transgenic animals
Mammary gland
Milk
Protein expression
Rabbit
Rat
Mouse
Livestock
Issue Date22-Jul-2005
PublisherCambridge University Press
CitationJournal of Dairy Research 72(S1): 113-119 (2005)
AbstractFor 10 years, the regulatory regions of the mouse and rabbit whey acidic protein gene have been used to express heterologous proteins in the milk of transgenic mice, as well as to produce pharmaceutical proteins, on a large scale, in the milk of transgenic livestock. To date, a broad range of expression levels have been detected, and elucidation of the structure-function relationship in these regulatory regions might help to achieve high levels of expression, reproducibly. An extended 5′ regulatory region (17·6 kb v. 6·3 kb) of the rabbit whey acidic promoter resulted in an increased frequency of rabbit whey acidic protein expression in transgenic mice. However, the expression levels were low compared with the high expression levels achieved in both transgenic mice and rabbits using the heterologous κ-casein in the 6·3 kb rabbit whey acidic protein 5′ regulatory region. These results underline the importance of the 3’ downstream regulatory regions, which still need to be better characterized in the whey acidic protein gene.
Description7 pages.-- PMID: 16180729 [PubMed].
Publisher version (URL)http://dx.doi.org/10.1017/S0022029905001111
URIhttp://hdl.handle.net/10261/8964
DOI10.1017/S0022029905001111
ISSN0022-0299
Appears in Collections:(CNB) Artículos

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