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Liquid chromatography-diode array detection to study the metabolism of glufosinate in Triticum aestivum T-590 and influence of the genetic modification on its resistance

AuthorsRojano-Delgado, Antonia María; Priego Capote, Feliciano; Barro Losada, Francisco ; Luque de Castro, María Dolores; Prado, R. del
KeywordsTriticum aestivum
GMO crops
Issue DateDec-2013
CitationPhytochemistry 96: 117-122 (2013)
AbstractThe resistance to glufosinate of two lines - genetically modified (GM) and unmodified (T-590 and T-549, respectively) - of Triticum aestivum has been studied. In the GM line, the bar gene was introduced to increase the resistance to glufosinate. Experiments in a controlled growth chamber showed that line T-590 presented a high resistance to glufosinate with an ED50 value of 478.59 g active ingredient per hectare (g ai ha-1) versus 32.65 g ai ha-1 for line T-549. The activity of glutamine synthetase (GS) in leaf extracts from both lines was investigated. The I50 for line T-590 was 694.10 μM glufosinate versus 55.46 μM for line T-549, with a resistance factor of 12.51. Metabolism studies showed a higher and faster penetration of glufosinate in line T-549 than in line T-590. LC-TOF/MS analysis of glufosinate metabolism at 48 h after herbicide treatment (300 g ai ha -1) revealed an 83.4% conversion of the herbicide (66.5% in N-acetyl-glufosinate metabolite), while in line T-549 conversion of the herbicide was about 40% (0% to N-acetyl-glufosinate). These results suggest that metabolism of glufosinate by the bar gene is a key mechanism of resistance in line T-590 that explains such high levels of herbicide tolerated by the plant, together with other mechanisms due to unmodified pathway, absorption and loss of glufosinate affinity for its target site.© 2013 Elsevier Ltd. All rights reserved.
Identifiersdoi: 10.1016/j.phytochem.2013.10.008
issn: 0031-9422
Appears in Collections:(IAS) Artículos
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