English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/89253
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Chitosan-gelatin biopolymers as carrier substrata for limbal epithelial stem cells

AuthorsMata, Ana de la; Nieto-Miguel, Teresa; López-Paniagua, Marina; Galindo, Sara; Aguilar, María Rosa ; García-Fernández, Luis ; Gonzalo, Sandra; Vázquez Lasa, Blanca; San Román, Julio ; Corrales, Rosa María; Calonge, Margarita
Issue Date2013
PublisherKluwer Academic Publishers
CitationJournal of Materials Science: Materials in Medicine 24: 2819- 2829 (2013)
AbstractThe aim of this work was to evaluate semi-synthetic biopolymers based on chitosan (CH) and gelatin (G) as potential in vitro carrier substrata for human limbal epithelial cells (hLECs). To that end, human corneal epithelial cells (HCE) were cultured onto different CH-G membranes. None of the polymers were cytotoxic and cell proliferation was higher when CH was functionalized with G. Expression levels of corneal epithelial markers (K3, K12, E-caherin, desmoplakin, and zonula occludens (ZO)-1) were better maintained in HCE cells grown on CH-G 20:80 membranes than other proportions. Consequently, CH-G 20:80 was chosen for the subsequent expansion of hLECs. Cells derived from limbal explants were successfully expanded on CH-G 20:80 membranes using a culture medium lacking components of non-human animal origin. The expression levels found for corneal (K3 and K12) and limbal epithelial stem cells (K15) specific markers were similar to or higher than those found in limbal cells grown onto the control substratum. Our results demonstrate that CH-G 20:80 membranes are suitable for the expansion and maintenance of stem cells derived from the limbal niche. These results strongly support the use of polymers as alternative substrata for the transplantation of cultivated limbal cells onto the ocular surface. © 2013 Springer Science+Business Media New York.
URIhttp://hdl.handle.net/10261/89253
DOI10.1007/s10856-013-5013-3
Identifiersdoi: 10.1007/s10856-013-5013-3
issn: 0957-4530
e-issn: 1573-4838
Appears in Collections:(ICTP) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.