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Title

Characterization of thirty two microsatellite loci for three Atlanto-Mediterranean echinoderm species

AuthorsGarcía-Cisneros, Álex ; Valero-Jiménez, Claudio; Palacín, Cruz ; Pérez-Portela, R.
KeywordsSea urchin
Pyrosequencing
Inbreeding
Clonality
Conservation
Starfish
Issue Date2013
PublisherSpringer
CitationConservation Genetics Resources (5) : 749-753 (2013)
AbstractThirty two microsatellites were optimized from 454 pyrosequencing libraries for three Atlanto-Mediterranean echinoderms: Coscinasterias tenuispina, Echinaster sepositus and Arbacia lixula. We observed different frequency of microsatellite types (di-, tri-, tetra- and pentanucleotide) throughout the genome of the species, but no significant differences were observed in allele richness among different microsatellite repeats. No loci showed linkage disequilibrium. Heterozygosity deficit and departure from Hardy–Weinberg equilibrium were observed for some loci, in two species, probably due to high levels of inbreeding. Heterozygosity excess observed in C. tenuispina could be explained by selection against homozygotes and/ or outcrossing.
Description5 páginas, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1007/s12686-013-9897-5
URIhttp://hdl.handle.net/10261/88706
DOI10.1007/s12686-013-9897-5
ISSN1877-7252
E-ISSN1877-7260
Appears in Collections:(CEAB) Artículos
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