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Closed Access item Differential CD4+ versus CD8+ T-Cell Responses Elicited by Different Poxvirus-Based Human Immunodeficiency Virus Type 1 Vaccine Candidates Provide Comparable Efficacies in Primates

Authors:Mooij, Petra
Balla-Jhagjhoorsingh, Sunita S.
Koopman, Gerrit
Beenhakker, Niels
Van Haaften, Patricia
Baak, Ilona
Nieuwenhuis, Ivonne G.
Kondova, Ivanela
Wagner, Ralf
Wolf, Hans
Gómez, Carmen E.
Nájera García, José Luis
Jiménez, Victoria
Esteban, Mariano
Heeney, Jonathan L.
Keywords:Immune response enhancement, Poxvirus vectors, Human immunodeficiency virus (HIV), Vaccine candidates, New York vaccinia virus (NYVAC), Modified vaccinia virus Ankara (MVA), CD4+/CD8+ T-cell immune response, Indian rhesus macaques
Issue Date:9-Jan-2008
Publisher:American Society for Microbiology
Citation:Journal of Virology 82(6): 2975-2988 (2008)
Abstract:Poxvirus vectors have proven to be highly effective for boosting immune responses in diverse vaccine settings. Recent reports reveal marked differences in the gene expression of human dendritic cells infected with two leading poxvirus-based human immunodeficiency virus (HIV) vaccine candidates, New York vaccinia virus (NYVAC) and modified vaccinia virus Ankara (MVA). To understand how complex genomic changes in these two vaccine vectors translate into antigen-specific systemic immune responses, we undertook a head-to-head vaccine immunogenicity and efficacy study in the pathogenic HIV type 1 (HIV-1) model of AIDS in Indian rhesus macaques. Differences in the immune responses in outbred animals were not distinguished by enzyme-linked immunospot assays, but differences were distinguished by multiparameter fluorescence-activated cell sorter analysis, revealing a difference between the number of animals with both CD4+ and CD8+ T-cell responses to vaccine inserts (MVA) and those that elicit a dominant CD4+ T-cell response (NYVAC). Remarkably, vector-induced differences in CD4+/CD8+ T-cell immune responses persisted for more than a year after challenge and even accompanied antigenic modulation throughout the control of chronic infection. Importantly, strong preexposure HIV-1/simian immunodeficiency virus-specific CD4+ T-cell responses did not prove deleterious with respect to accelerated disease progression. In contrast, in this setting, animals with strong vaccine-induced polyfunctional CD4+ T-cell responses showed efficacies similar to those with stronger CD8+ T-cell responses.
Description:14 pages, 8 figures.-- PMID: 18184713 [PubMed].-- Supplementary material available: Table S1: DNA sequence of MVA-89.6p-SIVgpn in the TK viral locus.-- Printed version published on Mar 2008.
Publisher version (URL):http://dx.doi.org/10.1128/JVI.02216-07
URI:http://hdl.handle.net/10261/8834
ISSN:0022-538X
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