1. DIGITAL.CSIC
  2. Ciencias Agrarias
  3. Instituto de Agrobiotecnología (IDAB)
  4. (IDAB) Artículos
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/86733
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE

Comparte tu historia
de Acceso Abierto
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Campo DC Valor Lengua/Idioma
dc.contributor.authorAndrés, Ximena de-
dc.contributor.authorRamírez, Hugo-
dc.contributor.authorSan Román, Beatriz-
dc.contributor.authorGlaria, Idoia-
dc.contributor.authorCrespo, Helena-
dc.contributor.authorJáuregui, Paula-
dc.contributor.authorAndrés, Damián F. de-
dc.contributor.authorReina, Ramsés-
dc.contributor.authorAmorena Zabalza, Beatriz-
dc.date.accessioned2013-11-14T12:41:16Z-
dc.date.available2013-11-14T12:41:16Z-
dc.date.issued2013-04-15-
dc.identifierdoi: 10.1016/j.vetimm.2012.12.017-
dc.identifierissn: 0165-2427-
dc.identifier.citationVeterinary Immunology and Immunopathology 152(3-4): 277-288 (2013)-
dc.identifier.urihttp://hdl.handle.net/10261/86733-
dc.descriptionAndrés, Ximena de et al.-
dc.description.abstractA single broadly reactive standard ELISA is commonly applied to control small ruminant lentivirus (SRLV) spread, but type specific ELISA strategies are gaining interest in areas with highly prevalent and heterogeneous SRLV infections. Short (15-residue) synthetic peptides (n= 60) were designed in this study using deduced amino acid sequence profiles of SRLV circulating in sheep from North Central Spain and SRLV described previously. The corresponding ELISAs and two standard ELISAs were employed to analyze sera from sheep flocks either controlled or infected with different SRLV genotypes. Two outbreaks, showing SRLV-induced arthritis (genotype B2) and encephalitis (genotype A), were represented among the infected flocks. The ELISA results revealed that none of the assays detected all the infected animals in the global population analyzed, the assay performance varying according to the genetic type of the strain circulating in the area and the test antigen. Five of the six highly reactive (57-62%) single peptide ELISAs were further assessed, revealing that the ELISA based on peptide 98M (type A ENV-SU5, consensus from the neurological outbreak) detected positives in the majority of the type-A specific sera tested (Se: 86%; Sp: 98%) and not in the arthritic type B outbreak. ENV-TM ELISAs based on peptides 126M1 (Se: 82%; Sp: 95%) and 126M2 0,65 0.77 (Se: 68%; Sp: 88%) detected preferentially caprine arthritis encephalitis (CAEV, type B) and visna/maedi (VMV, type A) virus infections respectively, which may help to perform a preliminary CAEV vs. VMV-like typing of the flock. The use of particular peptide ELISAs and standard tests individually or combined may be useful in the different areas under study, to determine disease progression, diagnose/type infection and prevent its spread. © 2013 Elsevier B.V.-
dc.description.sponsorshipFunded by CICYT (AGL2010-22341-C04-01) and Government of Navarra IIQ010449.RI1 and IIQ14064.RI1. H. Ramírez was supported by PASPA program, UNAM and Public University of Navarra. R. Reina was supported by CSIC JAE-Doc contract.-
dc.language.isoeng-
dc.publisherElsevier-
dc.rightsopenAccess-
dc.titleAn insight into a combination of ELISA strategies to diagnose small ruminant lentivirus infections-
dc.typeartículo-
dc.identifier.doi10.1016/j.vetimm.2012.12.017-
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.vetimm.2012.12.017-
dc.embargo.terms2014-04-15-
dc.date.updated2013-11-14T12:41:16Z-
dc.description.versionPeer Reviewed-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeartículo-
Aparece en las colecciones: (IDAB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato
insight_combination_ELISA_Andres.pdf259,67 kBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

37
checked on 10-abr-2024

WEB OF SCIENCETM
Citations

32
checked on 26-feb-2024

Page view(s)

429
checked on 23-abr-2024

Download(s)

528
checked on 23-abr-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.