Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/85871
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Genetic Evidence for p75NTR-Dependent Tetraploidy in Cortical Projection Neurons from Adult Mice |
Autor: | López-Sánchez, Noelia CSIC ORCID; Frade López, José María CSIC ORCID | Fecha de publicación: | 2013 | Editor: | Society for Neuroscience | Citación: | Journal of Neuroscience 33: 7488-7500 (2013) | Resumen: | A subpopulation of chick retinal projection neurons becomes tetraploid during development, an event prevented by blocking antibodies against p75 neurotrophin receptor (p75(NTR)). We have used an optimized flow cytometric assay, based on the analysis of unfixed brain cell nuclei, to study whether p75(NTR)-dependent neuronal tetraploidization takes place in the cerebral cortex, giving rise to projection neurons as well. We show that 3% of neurons in both murine neocortex and chick telencephalic derivatives are tetraploid, and that in the mouse ~85% of these neurons express the immediate early genes Erg-1 and c-Fos, indicating that they are functionally active. Tetraploid cortical neurons (65-80%) express CTIP2, a transcription factor specific for subcortical projection neurons in the mouse neocortex. During the period in which these neurons are born, p75(NTR) is detected in differentiating neurons undergoing DNA replication. Accordingly, p75(NTR)-deficient mice contain a reduced proportion of both NeuN and CTIP2-positive neocortical tetraploid neurons, thus providing genetic evidence for the participation of p75(NTR) in the induction of neuronal tetraploidy in the mouse neocortex. In the striatum tetraploidy is mainly associated with long-range projection neurons as well since ~80% of tetraploid neurons in this structure express calbindin, a marker of neostriatal-matrix spiny neurons, known to establish long-range projections to the substantia nigra and globus pallidus. In contrast, only 20% of tetraploid cortical neurons express calbindin, which is mainly expressed in layers II-III, where CTIP2 is absent. We conclude that tetraploidy mainly affects long-range projection neurons, being facilitated by p75(NTR) in the neocortex. | URI: | http://hdl.handle.net/10261/85871 | DOI: | 10.1523/JNEUROSCI.3849-12.2013 | Identificadores: | doi: 10.1523/JNEUROSCI.3849-12.2013 issn: 0270-6474 |
Aparece en las colecciones: | (IC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
JNeuroscience,2013,Frade.pdf | 6,83 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
19
checked on 24-abr-2024
SCOPUSTM
Citations
30
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
28
checked on 25-feb-2024
Page view(s)
318
checked on 24-abr-2024
Download(s)
263
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.