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dc.contributor.authorGutiérrez-Carbonell, Elaín-
dc.contributor.authorLattanzio, Giuseppe-
dc.contributor.authorSagardoy Calderón, Ruth-
dc.contributor.authorRodríguez-Celma, Jorge-
dc.contributor.authorRíos Ruiz, Juan José-
dc.contributor.authorMatros, Andrea-
dc.contributor.authorAbadía Bayona, Anunciación-
dc.contributor.authorAbadía Bayona, Javier-
dc.contributor.authorLópez-Millán, Ana Flor-
dc.date.accessioned2013-10-25T08:08:01Z-
dc.date.available2013-10-25T08:08:01Z-
dc.date.issued2013-12-
dc.identifier.citationGutiérrez-Carbonell E, Lattanzio G, Sagardoy R, Rodríguez-Celma J, Ríos JJ, Matros A, Abadía A, Abadía J, López-Millán AF. Changes induced by zinc toxicity in the 2-DE protein profile of sugar beet roots. Journal of Proteomics 94: 149-161 (2013)es_ES
dc.identifier.issn1874-3919-
dc.identifier.urihttp://hdl.handle.net/10261/84970-
dc.description13 Págs., 5 Figs., 1 Tabl.es_ES
dc.description.abstractChanges induced by three levels of Zn toxicity in the root proteome from Beta vulgaris were studied by two dimensional gel electrophoresis. 320 spots were consistently detected and 5, 5 and 11% of them showed significant changes in relative abundance as a result of the 50, 100 and 300. μM Zn treatments, respectively, when compared to controls (1.2. μM Zn). Forty-four spots had consistent changes between all treatments, and 93% were identified. At low and mild Zn excess, the complex I of the mitochondrial transport chain and the oxidative phosphorylation were mildly impaired, and an effort to compensate this effect by increasing glycolysis was observed. At high Zn excess, a general metabolism shutdown occurred, as denoted by decreases in the aerobic respiration and by an impairment of the defense systems against oxidative stress. Accordingly, lipid peroxidation increased as Zn supply increased. This study suggests that metabolic changes at high Zn supply reflect cell death, while changes at low and mild Zn supplies may rather explain the metabolic reprogramming occurring upon Zn toxicity. Results also suggest that Zn competition with divalent ions including Fe may contribute to many of the Zn toxicity symptoms, especially at low and moderate Zn supplies. Biological significance: Results in this work provide a comprehensive overview of the effects of Zn toxicity in roots of sugar beet plants. Effects at low and mild Zn excess are similar and reflect changes in the metabolism aimed to overcome this heavy metal stress, whereas effects at high Zn supply indicate a general shutdown of the metabolism and cell death. Our results indicate that Zn toxicity elicits major impairments in the oxidative stress defense systems, possibly due to Zn competition with divalent cations including Fe, in spite that Zn is not a redox active element by itself.es_ES
dc.description.sponsorshipThis work is supported by the Spanish Ministry of Economy and Competitivity (MINECO; projects AGL2010-16515 and AGL2012-31988, co-financed with FEDER) and the Aragón Government (Group A03)es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.subjectRootes_ES
dc.subjectSugar beetes_ES
dc.subjectTwo-dimensional gel electrophoresises_ES
dc.subjectZinc toxicityes_ES
dc.titleChanges induced by zinc toxicity in the 2-DE protein profile of sugar beet rootses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.jprot.2013.09.002-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jprot.2013.09.002es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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