English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/84853
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Characterization of the 3′:5′ ratio for reliable determination of RNA quality

AuthorsDie, José V. ; Obrero, Ángeles; González-Verdejo, Clara Isabel; Román, Belén
Issue Date15-Dec-2011
PublisherAcademic Press
CitationAnalytical Biochemistry 419(2): 336-338 (2011)
AbstractDetermination of RNA quality is a critical first step in obtaining meaningful gene expression data. The PCR-based 3′:5′ assay is an RNA quality assessment tool. This assay is a simple, fast, and low-cost method of selecting samples for further analysis. However, its practical applications are unexploited primarily because of the absence of an experimental threshold. We show that, by anchoring the 5′ assay a specific distance from the 3′ end of the sequence and by spacing the 3′ at a distance of a number of nucleotides, a cutoff determines whether a sample is suitable for downstream quantification studies. © 2011 Elsevier Inc. All rights reserved.
DescriptionAppendix A. Supplementary data
URIhttp://hdl.handle.net/10261/84853
DOI10.1016/j.ab.2011.08.012
Identifiersdoi: 10.1016/j.ab.2011.08.012
issn: 0003-2697
Appears in Collections:(IAS) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.