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Title

Activation of hatching in diapaused and quiescent Globodera pallida

AuthorsPalomares Rius, Juan E. ; Jones, John T.; Cock, Peter J.; Castillo, Pablo ; Blok, Vivian C.
KeywordsHatching
cGMP
Muscarinic pathway
Potato cyst nematodes
Root diffusate
Issue DateApr-2013
PublisherCambridge University Press
CitationParasitology 140(04): 445-454 (2013)
AbstractThe potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis are major pests of potatoes. The G. pallida (and G. rostochiensis) life cycle includes both diapause and quiescent stages. Nematodes in dormancy (diapause or quiescent) are adapted for long-term survival and are more resistant to nematicides. This study analysed the mechanisms underlying diapause and quiescence. The effects of several compounds (8Br-cGMP, oxotremorine and atropine) on the activation of hatching were studied. The measurements of some morphometric parameters in diapaused and quiescent eggs after exposure to PRD revealed differences in dorsal gland length, subventral gland length and dorsal gland nucleolus. In addition, the expression of 2 effectors (IVg9 and cellulase) was not induced in diapaused eggs in water or PRD, while expression was slightly induced in quiescent eggs. Finally, we performed a comparative study to identify orthologues of C. elegans diapause related genes in plant-parasitic nematodes (G. pallida, Meloidogyne incognita, M. hapla and Bursaphelenchus xylophilus). This analysis suggested that it was not possible to identify G. pallida orthologues of the majority of C. elegans genes involved in the control of dauer formation. All these data suggest that G. pallida may use different mechanisms to C. elegans in regulating the survival stage.
Publisher version (URL)http:dx.doi.org/10.1017/S0031182012001874
URIhttp://hdl.handle.net/10261/83101
DOI10.1017/S0031182012001874
ISSN0031-1820
E-ISSN1469-8161
Appears in Collections:(IAS) Artículos
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