English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/8286
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Topology of evolving, mutagenized viral populations: quasispecies expansion, compression, and operation of negative selection

AuthorsOjosnegros Martos, Samuel ; Agudo, Rubén ; Sierra García, Macarena ; Briones, Carlos ; Sierra, Saleta; González-López, Claudia; Domingo, Esteban ; Cristina, Juan
KeywordsVirus
Mutations
Partition Analysis of Quasispecies
PAQ
Issue Date17-Jul-2008
PublisherBioMed Central
CitationBMC Evolutionary Biology 2008, 8:207
AbstractBackground The molecular events and evolutionary forces underlying lethal mutagenesis of virus (or virus extinction through an excess of mutations) are not well understood. Here we apply for the first time phylogenetic methods and Partition Analysis of Quasispecies (PAQ) to monitor genetic distances and intra-population structures of mutant spectra of foot-and-mouth disease virus (FMDV) quasispecies subjected to mutagenesis by base and nucleoside analogues. Results Phylogenetic and PAQ analyses have revealed a highly dynamic variation of intrapopulation diversity of FMDV quasispecies. The population diversity first suffers striking expansions in the presence of mutagens and then compressions either when the presence of the mutagenic analogue was discontinued or when a mutation that decreased sensitivity to a mutagen was selected. The pattern of mutations found in the populations was in agreement with the behavior of the corresponding nucleotide analogues with FMDV in vitro. Mutations accumulated at preferred genomic sites, and dn/ds ratios indicate the operation of negative (or purifying) selection in populations subjected to mutagenesis. No evidence of unusually elevated genetic distances has been obtained for FMDV populations approaching extinction. Conclusion Phylogenetic and PAQ analysis provide adequate procedures to describe the evolution of viral sequences subjected to lethal mutagenesis. These methods define the changes of intra-population structure more precisely than mutation frequencies and Shannon entropies. PAQ is very sensitive to variations of intrapopulation genetic distances. Strong negative (or purifying) selection operates in FMDV populations subjected to enhanced mutagenesis. The quantifications provide evidence that extinction does not imply unusual increases of intrapopulation complexity, in support of the lethal defection model of virus extinction.
DescriptionAdditional files: Additional file 1: Neighbour-joining, maximum likelihood and maximum parsimony analysis of populations RAp35 and RA0p35. Additional file 2: Maximum likelihood phylogenetic analysis of mutagenized populations of FMDV. Additional file 3: Standard errors.
Publisher version (URL)http://dx.doi.org/10.1186/1471-2148-8-207
URIhttp://hdl.handle.net/10261/8286
DOI10.1186/1471-2148-8-207
ISSN1471-2148
Appears in Collections:(CBM) Artículos
(CAB) Artículos
Files in This Item:
File Description SizeFormat 
1471-2148-8-207.pdf1,69 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.