Expression and distribution of cytoskeleton markers in Gilthead seabream (Sparus aurata L.)

Abstract

Introduction: Protein filaments that form part of the cytoskeleton constitute an internal dynamic framework which carries out vital cell functions. However, lack of species-specific antibodies is a limiting factor in conducting a detailed fish tissue study. This implies the use of antibodies developed in other species, which possess significant cross-reactivity. The objective of our study was to standardize immunohistochemical techniques in gilthead seabream (Sparus aurata L.) tissues to detect several cytoskeleton molecules including some involved in the cell-to-cell adhesions. Material and methods: We used antibodies against anti-human: E-cadherin; annexin V; occludin and claudin. Samples from digestive tract, pancreas, liver, thymus and kidney fixed in Bouin¿s for 24 hours and embedded in paraffin were employed. Positive and negative controls were used for each antibody. Results: Trials showed positive spotted expression in epithelial cell membranes especially of digestive mucosa, when using E-cadherin and annexin V antibodies. The later also marked rodlet cells of specific digestive tube sections. Presently, no satisfactory immunoreactivity was achieved with the occludinor laudin antibodies. Conclusion: This data contributes to enhance knowledge regarding fish tissue structure providing a useful tool for a more accurate physiological and histopathological studies.