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Título: | Constitutive COX-2 activity in cardiomyocytes confers permanent cardioprotection: Constitutive COX-2 expression and cardioprotection |
Autor: | Mollá, Belén CSIC ORCID; Través, Paqui G. CSIC; Martín-Sanz, Paloma CSIC ORCID ; Boscá, Lisardo CSIC ORCID CVN ; Casado, Marta CSIC ORCID ; García-Dorado, David | Fecha de publicación: | 2009 | Editor: | Elsevier | Citación: | Journal of Molecular and Cellular Cardiology 46(2): 160-168 (2009) | Resumen: | Different lines of evidence suggest that inhibition of COX-2 activity exacerbates reperfusion injury, but direct data showing beneficial effects of increased COX-2 activity are lacking. The aim of this study was to determine the effect of constitutive expression of COX-2 on cardiomyocyte tolerance to ischemia-reperfusion injury. We generated a transgenic mouse (B6D2-Tg (MHC-PTGS2)17Upme) that constitutively expresses functional human COX-2 in cardiomyocytes under the control of α-myosin heavy chain promoter. COX-2 expression was confirmed by immunoblotting and by increased levels of PGE2 and PGI2 in myocardium. Histological and echocardiographic analysis revealed no differences in the phenotype of transgenic mice (TgCOX-2) with respect to wild type (Wt) mice. Tolerance to ischemia-reperfusion injury was analysed in a Langendorff system. Reperfused TgCOX-2 hearts after 40 min of ischemia improved functional recovery (32.9 ± 6.2% vs. 9.45 ± 4.4%, P = 0.004) and reduced cell death assessed by LDH release (43% of reduction, P < 0.001) and triphenyltetrazolium staining (41% of reduction, P = 0.002). Cardioprotection was not further increased by ischemic preconditioning. Pretreatment of mice with the COX-2 inhibitor DFU attenuated cardioprotection with a correlation between myocardial PGE2 levels and the extent of cell death. NMR spectroscopy showed a marked reduction in arachidonic acid (AA) content in TgCOX-2 hearts. Both, DFU pretreatment and perfusion of TgCOX-2 hearts with AA increased myocardial AA to values similar to those measured in Wt hearts and reversed cardioprotection. We conclude that constitutive expression of COX-2 in cardiomyocytes confers a permanent cardioprotective state against reperfusion injury. Increased PGE2 synthesis and reduced AA content could explain this effect. | URI: | http://hdl.handle.net/10261/82616 | DOI: | 10.1016/j.yjmcc.2008.11.011 | Identificadores: | doi: 10.1016/j.yjmcc.2008.11.011 issn: 0022-2828 e-issn: 1095-8584 |
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