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Are peroxiredoxin genes predictive biomarkers of health and welfare in farmed fish? Answers from gilthead sea bream (Sparus aurata) exposed to parasitic, dietary and confinement stressors

AuthorsPérez-Sánchez, Jaume ; Bermejo-Nogales, Azucena ; Calduch-Giner, Josep A. ; Kaushik, Sadasivam; Sitjà-Bobadilla, Ariadna
Issue Date2011
PublisherPennsylvania State University
Citation1st Annual World Congress of Microbes (2011)
AbstractFarmed fish are constantly exposed to different type of stressors, including a higher risk of acquisition of parasitic infections due to high density culture conditions, inadequate diets and handling. Enteromyxum leei is a wide-spread enteric myxosporean parasite causing heavy economic losses in Mediterranean sparid farms. There are no preventive or curative treatments for this enteromyxosis, which causes severe desquamative enteritis and usually leads to death of fish. PRDXs are antioxidant enzymes that catalyze the reduction of peroxides and alkyl peroxides. As part of an European Project, AQUAMAX (www.aquamaxip.eu), extensive BLAST searches on the gilthead sea bream (GSB, Sparus aurata) cDNA database (www.sigenae.org/iats) and sequence completion by RT-PCR unequivocally identified six PRDXs, which were similar to those already indentified in mammals. The analysis of 11 tissues by real-time PCR evidenced an ubiquitous expression that was tissue-specific for each PRDX isoform. Then, juvenile GSB were challenged with i) E. leei by effluent transmission during 113 days, ii) a vegetable oil (VO) diet with reduced levels of essential fatty acids and iii) prolonged exposure to high rearing densities. Liver and head kidney (HK) were chosen as target tissues to analyze the regulation of the six PRDXs. PRDX transcripts were highly and most constitutively expressed in the liver, and were not affected by dietary intervention or rearing density. In contrast, HK was highly sensitive to the different experimental challenges: significantly lower values were found for PRDX5 under the three challenge conditions, for PRDX6 in parasitized and high density reared fish and for PRDX1 in parasitized and VO-fed fish. PRDX2, 3 and 5 were respectively decreased only in VO-fed fish, high density and parasitized animals. These findings highlight the role of PRDXs as integrative and highly predictive biomarkers of health and welfare in GSB.
DescriptionPonencia presentada en el 1st Annual World Congress of Microbes celebrado en China del 30 de julio al 1 de agosto de 2011
Appears in Collections:(IATS) Comunicaciones congresos
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