Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/81628
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | CRISPR-Cas systems preferentially target the leading regions of MOBF conjugative plasmids |
Autor: | Cruz, Fernando de la CSIC ORCID; Brouns, Stan J. J. | Fecha de publicación: | 2013 | Editor: | Landes Bioscience | Citación: | RNA Biology 10(5): 749-761 (2013) | Resumen: | Most prokaryotes contain CRISPR-Cas immune systems that provide protection against mobile genetic elements. We have focused on the ability of CRISPR-Cas to block plasmid conjugation, and analyzed the position of target sequences (protospacers) on conjugative plasmids. The analysis reveals that protospacers are non-uniformly distributed over plasmid regions in a pattern that is determined by the plasmid's mobilization type (MOB). While MOBP plasmids are most frequently targeted in the region entering the recipient cell last (lagging region), MOBF plasmids are mostly targeted in the region entering the recipient cell first (leading region). To explain this protospacer distribution bias, we propose two mutually non-exclusive hypotheses: (1) spacers are acquired more frequently from either the leading or lagging region depending on the MOB type (2) CRISPR-interference is more efficient when spacers target these preferred regions. To test the latter hypothesis, we analyzed Type I-E CRISPR-interference against MOBF prototype plasmid F in Escherichia coli. Our results show that plasmid conjugation is effectively inhibited, but the level of immunity is not affected by targeting the plasmid in the leading or lagging region. Moreover, CRISPR-immunity levels do not depend on whether the incoming single-stranded plasmid DNA, or the DNA strand synthesized in the recipient is targeted. Our findings indicate that single-stranded DNA may not be a target for Type I-E CRISPR-Cas systems, and suggest that the protospacer distribution bias might be due to spacer acquisition preferences. © 2013 Landes Bioscience. | URI: | http://hdl.handle.net/10261/81628 | DOI: | 10.4161/rna.24202 | Identificadores: | doi: 10.4161/rna.24202 issn: 1547-6286 e-issn: 1555-8584 |
Aparece en las colecciones: | (IBBTEC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
16
checked on 29-feb-2024
SCOPUSTM
Citations
27
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
25
checked on 28-feb-2024
Page view(s)
285
checked on 24-abr-2024
Download(s)
100
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.